酶解骨粉对低钙大鼠的补钙功效研究  

Effect of Enzymatic Hydrolyzed Bone Powder on Calcium Supplement for Low Calcium Rats

作　　者：李玉娟[1] 肖楠 刘珊 刘菲菲 隋岩 LI Yujuan;XIAO Nan;LIU Shan;LIU Feifei;SUI Yan(School of Life Science,Beijing Institute of Technology,Beijing 100081,China;TianQi Biotechnology Company Limited,Chifeng,Inner Mongolia 024000,China)

机构地区：[1]北京理工大学生命学院,北京100081 [2]内蒙古天奇生物科技有限公司,内蒙古赤峰市024000

出　　处：《北京理工大学学报》2022年第1期105-110,共6页Transactions of Beijing Institute of Technology

基　　金：国家自然科学基金资助项目(81973572)。

摘　　要：为研究酶解骨粉对低钙大鼠的补钙效果,经过六周低钙饲养SD大鼠构建低钙模型,连续四周分别灌胃给予低钙大鼠200、400和800 mg/kg酶解骨粉溶液.对大鼠股骨进行HE染色分析,测定股骨力学指标、骨密度、干重灰重、骨钙和骨磷含量,并测定钙的表观吸收率.实验结果发现模型组大鼠骨小梁显著减少,骨髓腔明显扩大.给予酶解骨粉后大鼠骨小梁数量增加,骨髓腔面积减小.高剂量酶解骨粉组大鼠的骨密度提高了14.1%,股骨钙磷含量和钙表观吸收率也显著升高(p<0.05).研究表明酶解骨粉能够促进低钙大鼠钙的吸收,提高大鼠骨密度,增加体内骨钙含量,或是一种潜在的补钙制剂.To investigate the effect of enzymatic hydrolyzed bone powder(EBP)on calcium supplement for low calcium rats.6-week low calcium diet was used to establish a low calcium model.EBP(200,400,800 mg/kg)was orally administered to low calcium rats respectively for 4 weeks.The femur of rats was analyzed by HE staining.The mechanical index,dry weight,ash weight,and bone mineral density of the femur were measured.Femur calcium content,femur phosphorus content and the apparent absorption rate of calcium were determined.Results show that the bone trabecula is significantly decreased and the bone marrow cavity is remarkably enlarged in low calcium rats.After administration of EBP,the number of bone trabecula has increased and the area of bone marrow cavity has decreased.The bone mineral density of rats in high dose EBP can enhance by 14.1%.Besides,the content of calcium and phosphorus in the femur and the apparent absorption rate of calcium can increase significantly(p<0.05).These findings suggest that EBP can promote calcium absorption,increase bone mineral density and bone calcium content in low calcium rats,being a potential calcium supplement.

关 键 词：补钙功效 酶解骨粉 骨密度 补钙制剂 

分 类 号：R917[医药卫生—药物分析学]