猪流行性腹泻病毒GDsg株的分离鉴定及遗传进化分析  

Isolation,Identification and Genetic Variation Analysis of Porcine Epidemic Diarrhea Virus GDsg Strain

作　　者：董建国 饶丹 陈明睿 何书海 焦凤超 陈斌 易本驰 黄立 赵攀登 DONG Jian-guo;RAO Dan;CHEN Ming-rui;HE Shu-hai;JIAO Feng-chao;CHEN Bin;YI Ben-chi;HUANG Li;ZHAO Pan-deng(School of Animal Husbandry and Medical Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,China;College of Veterinary Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China)

机构地区：[1]信阳农林学院牧医工程学院,河南信阳464000 [2]河南牧业经济学院动物医学院,河南郑州450046

出　　处：《中国兽医杂志》2021年第10期1-5,共5页Chinese Journal of Veterinary Medicine

基　　金：河南省科技攻关项目(212102110364);河南省高等学校青年骨干教师培养计划项目(教高[2020]354号);信阳农林学院重点培育学科项目(ZDXK201702);河南省重点研发与推广专项(202102110095)。

摘　　要：为了解广东地区某猪场猪流行性腹泻(PED)的流行情况,本试验通过病毒分离纯化、序列比对分析以确定疑似病料感染情况。结果显示:成功分离猪流行性腹泻病毒(PEDV),命名为GDsg株,该毒株属于变异株GIIa亚型分支,与经典株GIa亚型代表毒株CV777同源性最低,为96.6%;与经典株GIb亚型代表毒株SD-M同源性为97.0%;与变异株GIIa亚型代表毒株AJ1102同源性最高,为98.3%;与变异株GIIb亚型代表毒株CH-JX-1-2013同源性为98.1%。与经典株CV777相比,GDsg株S基因在中和抗原表位COE有6个氨基酸突变,在线性抗原表位SS6区间有1个氨基酸突变。本试验结果表明该猪场腹泻是由流行变异毒株GIIa亚型GDsg株引起的。This study isolated a porcine epidemic diarrhea virus(PEDV) strain(named GDsg strain) on Vero cells from small intestine samples of a suspected PEDV pig farm in Shaoguan city,Guangdong province,and showed that GDsg strain belonged to the GIIa subtype branch of the variant strain.Phylogenetic tree and homology alignment analyses revealed 96.6% nucleotide sequence homology with the classic strain GIa subtype representative strain CV777(lowest homology),97.0% nucleotide sequence homology with the classic strain GIb subtype representative strain SD-M,98.3% nucleotide sequence homology with the variant GIIa subtype strain AJ1102(highest homology),98.1% nucleotide sequence homology with the variant GIIb subtype strain CH-JX-1-2013.The amino acid sequence alignment indicated that the S gene of GDsg strain had 6 amino acid mutations in the neutralizing epitope COE and one amino acid mutation in the linear epitope SS6 in comparison to the classical strain CV777.These results suggested that GDsg strain,an endemic mutant strain GIIa subtype,was responsible for causing diarrhea in this Guangdong pig farm.

关 键 词：猪流行性腹泻病毒 分离鉴定 进化分析 同源性 

分 类 号：S855.3[农业科学—临床兽医学]