维生素C体外抑制SVCV入侵鲤鱼上皮瘤细胞的初步研究  

Preliminary Study on Vitamin C Inhibiting SVCV Invasion of Epithelioma Papulosum Cyprini In Vitro

作　　者：刘佳 卢玉婷[1] 刘芸娜 闫子豪 汪惠庆 李月红[1] LIU Jia;LU Yu-ting;LIU Yun-na;YAN Zi-hao;WANG Hui-qing;LI Yue-hong(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国兽医杂志》2021年第10期10-14,共5页Chinese Journal of Veterinary Medicine

基　　金：吉林省自然科学基金(20190201179JC);淡水鱼类发育生物学国家重点实验室项目(201901)。

摘　　要：为探究维生素C体外抑制鲤春病毒血症病毒(SVCV)侵染鲤鱼上皮瘤细胞(EPC)的能力,本试验构建了体外EPC模型,并运用MTT法、DAPI染色和活性氧测定等方法测定维生素C和SVCV对细胞活性的影响及维生素C对SVCV的抑制作用。结果显示,维生素C对SVCV体外侵染EPC具有显著的抑制作用(P<0.05),且与维生素C浓度呈正相关。本试验可为SVCV的防治提供新的途径。This study examined the ability of vitamin C to inhibit spring viremia of carp virus(SVCV) infection in epithelioma papulosum cyprini(EPC) in vitro.After construction of an in vitro EPC model,the effect of vitamin C and SVCV on cell viability was assessed by using MTT,DAPI staining and active oxygen determination.The results showed that vitamin C had a significant inhibitory effect on SVCV infection of EPC in vitro,which positively correlated with the concentration of vitamin C.This study provides a new approach for control and prevention of SVCV.

关 键 词：维生素C 鲤春病毒血症病毒 鲤鱼上皮细胞 抑制 

分 类 号：R631.1[医药卫生—外科学]