吸附NT-3的3D打印胶原蛋白/壳聚糖支架改善脊髓损伤后的运动功能  

3D printing collagen/chitosan scaffold adsorbed NT-3 improved locomotor function after spinal cord injury

作　　者：隋曌[1] 彭凤[2] 余凯 严小虎[3] 李英[4] 钟琳 刘晓银 Sui Zhao;Peng Feng;Yu Kai;Yan Xiaohu;Li Ying;Zhong Lin;Liu Xiaoyin(Department of Neurosurgery,First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,China;Department of Anesthesiology,First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,China;Department of Orthopaedics,First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,China;Department of Cardiothoracic Surgery,First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,China;Department of Hematology,First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,China;Department of Neurosurgery,West China Hospital,Sichuan University,Chengdu 610065,China)

机构地区：[1]成都医学院第一附属医院神经外科,成都610500 [2]成都医学院第一附属医院麻醉科,成都610500 [3]成都医学院第一附属医院骨科,成都610500 [4]成都医学院第一附属医院胸心外科,成都610500 [5]成都医学院第一附属医院血液科,成都610500 [6]四川大学华西医院神经外科,成都610041

出　　处：《中华神经创伤外科电子杂志》2021年第6期331-338,共8页Chinese Journal Of Neurotraumatic Surgery：Electronic Edition

摘　　要：目的探究移植吸附神经营养素-3(NT-3)的3D打印胶原蛋白/壳聚糖支架对于脊髓损伤后运动功能的改善作用。方法制备吸附NT-3的3D打印胶原蛋白/壳聚糖支架。将80只成年雌性SD大鼠(220~250 g)随机分为4组:假手术组、模型组、3D打印支架组和3D打印支架-因子组,每组20只。假手术组:暴露脊髓后直接缝合硬脊膜无损伤;模型组:只进行T10脊髓全横断损伤;3D打印支架组:T10脊髓全横断损伤后植入3D打印胶原蛋白/壳聚糖支架;3D打印支架-因子组:T10脊髓全横断损伤后植入吸附NT-3的3D打印胶原蛋白/壳聚糖支架。脊髓损伤后1~8周采用BBB评分和斜坡爬壁实验。脊髓损伤后8周行电生理分析,评估大鼠的运动功能;行弥散张量纤维束成像观察脊髓缺损部位恢复情况。结果(1)脊髓损伤后1~8周,相对于假手术组、模型组和3D打印支架组,植入吸附NT-3的3D打印胶原蛋白/壳聚糖支架可以显著提高脊髓损伤大鼠的BBB评分和斜坡角度(P<0.05);(2)脊髓损伤后8周,与3D打印支架组和模型组相比,3D打印支架-因子组展现出在左后肢和右后肢的运动诱发电位和体感诱发电位中振幅和潜伏期的改善(P<0.05);(3)脊髓损伤后8周弥散张量纤维束成像的图像证明3D打印支架-因子组的脊髓损伤区域的再生神经纤维数量明显多于3D打印支架组和模型组。结论吸附NT-3的3D打印胶原蛋白/壳聚糖支架能改善脊髓损伤后的运动功能,有潜力成为脊髓损伤修复创新和安全的方法。Objective To explore the effect of transplantation of 3D printing collagen/chitosan scaffold adsorbed neurotrophin-3(NT-3)on improving locomotor function after spinal cord injury(SCI).Methods The 3D printing collagen/chitosan scaffold adsorbed NT-3 was prepared.Eighty adult female SD rats(220-250 g)were randomly divided into four groups:sham operation group,model group,3D printing scaffold group and 3D printing scaffold-factor group,with 20 rats in each group.Sham operation group:the spinal cord was exposed to the spinal cord and directly sutured the endorachis without injury;Model group:only undergoes T10 spinal cord transection;3D printing scaffold group T10 spinal cord transection is implanted with 3D printing collagen/chitosan;3D printing scaffold-factor group:T10 spinal cord transection was implanted with 3D printing collagen/chitosan scaffold adsorbed by NT-3.1-8 weeks after SCI,the BBB score and the inclined-grid climbing test were used to evaluate the locomotor function of rats 8 weeks after SCI.Diffusion tensor fiber tract imaging was performed to observe the recovery of spinal cord defect at 8 weeks after SCI.Results(1)1-8 weeks after SCI,compared to sham operation group,model group and 3D printing scaffold group,implantation of 3D printing collagen/chitosan scaffold adsorbed by NT-3 could significantly improve the spinal cord BBB score and slope angle of injured rats(P<0.05).(2)8 weeks after SCI,compared with the 3D printing scaffold and model groups,the 3D printing scaffold-factor group showed improvements in amplitude and latency in the motor and somatosensory evoked potentials of the left and right hind limbs(P<0.05).(3)The image of diffusion tensor fiber bundle imaging at 8 weeks after SCI proved that the number of regenerated nerve fibers in the SCI area in 3D printing scaffold-factor group was significantly more than that of the 3D printing scaffold and model groups.Conclusion 3D printing collagen/chitosan scaffold adsorbed NT-3 can improve locomotor function after SCI,and has the potential to be

关 键 词：脊髓损伤 3D打印 胶原蛋白 壳聚糖 神经营养素-3 支架 运动功能 弥散张量纤维束成像 

分 类 号：R651.2[医药卫生—外科学] R318.08[医药卫生—临床医学]