草假单胞菌HT1在蚕豆根和茎的定殖特性及对内生细菌多样性的影响  

Colonization characteristics of Pseudomonas poae HT1 in roots and stems of faba bean and its effect on endophytic bacterial diversity

作　　者：张慧[1] 马连杰[1] 卢文才 余端[1] 杭晓宁[1] 廖敦秀[1] ZHANG Hui;MA Lianjie;LU Wencai;YU Duan;HANG Xiaoning;LIAO Dunxiu(Institute of Agricultural Resources and Environment,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China)

机构地区：[1]重庆市农业科学院农业资源与环境研究所,重庆401329

出　　处：《微生物学通报》2021年第12期4677-4687,共11页Microbiology China

基　　金：重庆市绩效激励引导专项(cqaas2019jx110,cqaas2020jxjl08);重庆市农发资金项目(NKY-2021AC002);重庆市良种创新项目(NKY-2021AB003)。

摘　　要：【背景】关于蚕豆内生生防细菌的定殖规律及其对内生细菌微生物多样性的研究鲜有报道。【目的】明确草假单胞菌HT1在蚕豆的定殖特性以及对根茎部内生细菌群落多样性的影响。【方法】采用抗生素标记法测定菌株HT1的定殖特性,利用高通量测序技术分析该菌灌根处理与对照处理蚕豆根茎部内生细菌的群落多样性。【结果】菌株HT1的定殖数量为根>茎>叶,呈先升后降的趋势,根部、茎部、叶部在第7天达到最大值,在第83天仍能检测到标记菌株。灌根处理降低了内生细菌的丰富度,提高了根内生细菌物种多样性;灌根处理组根部厚壁菌门、放线菌门、拟杆菌门丰度显著提高,茎部变形菌门的相对丰度有所升高但无显著影响;在属水平上,灌根处理后,根部如Romboutsia、Mitsuaria、乳杆菌属、德沃斯氏菌属等属的相对丰度明显升高,茎部假单胞菌、Muribaculaceae、Elstera、鞘氨醇单胞菌属等属的相对丰度明显升高。【结论】HT1菌株能在蚕豆植株体内定殖达83 d以上,并且可以影响蚕豆植株内部的微生物群落结构组成,提高相关微生物的相对丰度。[Background]There are few reports on the colonization of endophytic control bacteria in faba bean and the diversity of endophytic bacteria.[Objective]To investigate the colonization characteristics of Pseudomonas poae HT1 in faba bean and its effect on the diversity of endophytic bacterial community in the rhizome.[Methods]The colonization characteristics of strain HT1 were determined by antibiotic labeling method,and the community diversity of endophytic bacteria in root and stem of strain HT1 was analyzed by high-throughput sequencing technology.[Results]The number of colonization of strain HT1 was root>stem>leaf,which increased first and then decreased.The root,stem and leaf reached the maximum on the 7 th day,and the labeled strain could still be detected on the 83 rd day.Root irrigation reduced the richness of endophytic bacteria and increased species diversity of endophytic bacteria in roots.The abundances of Firmicutes,Actinobacteria,Bacteroidetes in roots were significantly increased in irrigated root group,while the relative abundances of Proteobacteria in stems were increased but had no significant effect.At the genus level,after root irrigation,the relative abundance of Romboutsia,Mitsuaria,Lactobacillus,Devosia and other genera increased significantly in the root,and the relative abundance of Pseudomonas,Muribaculaceae,Elstera,Sphingomonas in the stem increased significantly.[Conclusion]The strain HT1 could colonize in faba bean plants for more than 83 days,and could affect the composition of microbial community inside the plants and improve the relative abundance of related microorganisms.

关 键 词：草假单胞菌HT1 蚕豆 定殖 微生物群落结构 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]