江苏地区10株鹅源鸭疫里默氏杆菌的耐药性及致病性特征分析  

Drug resistance and pathogenicity analyses of 10 strains of Rimerella anatipestifer isolated from geese in Jiangsu

作　　者：董洪燕 张燕 王彦红[2] 吴植[1] 杨静静 王安平[1] 徐海 朱善元[1] 于圣青[4] DONG Hongyan;ZHANG Yan;WANG Yanhong;WU Zhi;YANG Jingjing;WANG Anping;XU Hai;ZHU Shanyuan;YU Shengqing(Jiangsu Agri-Animal Husbandry Vocational College,Taizhou,Jiangsu 225300,China;College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China;Jiangsu Guiliu Animal Husbandry Group Limited Company,Xuzhou,Jiangsu 221000,China;Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]扬州大学兽医学院,江苏扬州225009 [3]江苏桂柳牧业集团有限公司,江苏徐州221000 [4]中国农业科学院上海兽医研究所,上海200241

出　　处：《微生物学通报》2021年第12期4756-4764,共9页Microbiology China

基　　金：江苏省农业科技自主创新资金项目(CX(18)1004);泰州市科技支撑计划(农业)项目(TN201801);江苏现代农业产业技术体系集成创新中心(JATS[2020]345);江苏省高等学校自然科学研究重大项目(20KJA230004)。

摘　　要：【背景】鸭疫里默氏杆菌感染是危害水禽业最严重的细菌传染性病之一,鹅源鸭疫里默氏杆菌病的发病率有逐渐增高趋势,给养鹅业带来了巨大经济损失。【目的】为更好地预防控制鹅源鸭疫里默氏杆菌病、解决鹅养殖场临床用药问题及进一步探究鸭源和鹅源鸭疫里默氏杆菌之间的关系。【方法】对江苏地区的发病鹅进行鸭疫里默氏杆菌的分离培养、多重PCR方法鉴定及生化试验,并对分离获得的10株鸭疫里默氏杆菌进行药敏试验、血清型鉴定及雏鸭致病性试验。【结果】药敏试验结果显示,鹅源分离菌株对大观霉素、磺胺异噁唑、头孢曲松、头孢拉定、氟苯尼考最敏感,对新霉素、卡那霉素、丁胺卡那霉素、庆大霉素和克林霉素耐药性最高且多重耐药性严重。血清型鉴定表明,江苏地区分离的10株鹅源鸭疫里默氏杆菌主要以血清型2型为主。雏鸭致病性试验表明,鹅源鸭疫里默氏杆菌分离株均引起雏鸭不同程度发病,其中3株鹅源临床分离株经1×107 CFU/羽攻毒后可引起雏鸭100%的致死率。【结论】为鹅源鸭疫里默氏杆菌的预防控制、临床治疗及进一步研究鸭源和鹅源鸭疫里默氏杆菌之间的关系提供依据。[Background]Riemerella anatipestifer(RA) is one of the most serious bacterial diseases in water poultry industry.The incidence of Rimerella anatipestifer from geese is increasing,it also brings great economic losses in goose industry.[Objective]To better prevent Rimerella anatipestitis infection in the geese,solve the problems of clinical medication in goose farms and further clarify the relationship between the duck-and goose-origin of RA strains.[Methods]Bacteria were isolated from moribund or dead geese in Jiangsu province and identified by multiple polymerase chain reaction(PCR) and biochemical test.Ten RA strains isolated from the geese were tested for the drug susceptibility,serotypes and pathogenicity in ducks.[Results]Drug sensitivity test showed that majority of the 10 strains from geese were sensitive to mesomycin,sulfafurazole,ceftriaxone,cefradine and florfenicol,but resistance to neomycin,kanamycin,amikacin,gentamicin and clindamycin.All ten strains were multi-drug resistance to 15-25 tested drugs.Serotype 2 was the main serotype of 10 Rimerella anatipestifer isolated from geese in Jiangsu province.Pathogenicity tests on ducklings showed that all the Rimerella anatipestitis isolates from geese caused different degrees of disease in ducklings,and the mortality rate of 3 clinical isolates from geese after 1×107 CFU/feathering challenge was 100%.[Conclusion]This study provides a basis for the prevention,control and clinical treatment of Rimerella anatipestifer from geese in Jiangsu,as well as the further study of the relationship between Rimerella anatipestifer between the duck-and goose-origin of RA strains.

关 键 词：鹅源 鸭疫里默氏杆菌 药敏试验 雏鸭致病性试验 

分 类 号：S852.61[农业科学—基础兽医学]