猪流行性腹泻病毒S蛋白胞内区线性B细胞表位最小基序鉴定  

Identification of minimal motif of linear B cell epitope on the cytoplasmic domain of porcine epidemic diarrhea virus S protein

作　　者：张政中 董世娟[2,3] 司伏生 陈冰清[2,3] 谢春芳 李震[2,3] 李家奎 于瑞嵩[2,3] ZHANG Zhengzhong;DONG Shijuan;SI Fusheng;CHEN Bingqing;XIE Chunfang;LI Zhen;LI Jiakui;YU Ruisong(Department of Animal Science,Tibet Agricultural&Animal Husbandry University,Linzhi,Tibet 860000,China;Shanghai Engineering Research Center of Breeding Pig,Institute of Animal Husbandry and Veterinary Medicine,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106,China)

机构地区：[1]西藏农牧学院动物科学学院,西藏林芝860000 [2]上海种猪工程技术研究中心上海市农业科学院畜牧兽医研究所,上海201106 [3]上海市农业遗传育种重点实验室,上海201106

出　　处：《微生物学通报》2021年第12期4828-4835,共8页Microbiology China

基　　金：国家重点研发计划(2016YFD0500101);国家自然科学基金(32072838);上海市科技兴农重点攻关项目(2020-02-08-0012-F01478)。

摘　　要：【背景】S蛋白是猪流行性腹泻病毒(Porcine Epidemic Diarrhea Virus,PEDV)的主要结构蛋白和免疫原性蛋白,在前期的研究中,本课题组在S蛋白的胞内区鉴定到2个包含线性B细胞表位的短肽。【目的】鉴定PEDV S蛋白胞内区线性B细胞表位的最小基序。【方法】原核表达2个短肽的每次后移1个氨基酸的系列8肽,以兔抗S蛋白血清为一抗,通过Western Blot筛选阳性反应8肽,鉴定S蛋白胞内区线性B细胞表位的最小基序。【结果】S蛋白胞内区的2个包含线性B细胞表位的短肽共享一个表位,该表位的最小基序为^(1371)QPYE^(1374)。同源性分析显示该B细胞表位基序为保守性表位。【结论】确定了S蛋白胞内区线性B细胞表位的最小基序为^(1371)QPYE^(1374);S蛋白抗原表位的鉴定有助于提高对其结构和功能的理解。[Background]S protein is the main structural and immunogenic protein of PEDV.In our previous work,two linear B cell epitopes (BCE) containing short peptides were identified in the cytoplasmic domain of S protein.[Objective]To identify the minimal motif of the linear BCE were identified in the cytoplasmic domain of PEDV S protein.[Methods]Serial 8-mers,advancing one aa once on the short peptide,were prokaryotically expressed.Positive 8-mers were identified by Western Blot using rabbit anti-S serum as the primary antibody and the minimal motif of the linear BCE was identified within the cytoplasmic domain of S protein.[Results]The two short peptides within the cytoplasmic domain of S protein contained the same epitope,and the minimum motif of the epitope was ^(1371)QPYE^(1374).Homologous analysis showed that the BCE motif was conserved.[Conclusion]The minimum motif of the BCE within the cytoplasmic domain of S protein was determined as ^(1371)QPYE^(1374).Identification of antigen epitope on S protein helps to improve the understanding of its structure and function.

关 键 词：猪流行性腹泻病毒 S蛋白 胞内区 线性B细胞表位 最小基序鉴定 

分 类 号：S852.651[农业科学—基础兽医学]