Low-Cost and Scalable Platform with Multiplexed Microwell Array Biochip for Rapid Diagnosis of COVID-19  

作　　者：Yang Wang Kaiju Li Gaolian Xu Chuan Chen Guiqin Song Zaizai Dong Long Lin Yu Wang Zhiyong Xu Mingxia Yu Xinge Yu Binwu Ying Yubo Fan Lingqian Chang Jia Geng 

机构地区：[1]Beijing Advanced Innovation Center for Biomedical Engineering,Key Laboratory for Biomechanics and Mechanobiology,School of Biological Science and Medical Engineering,Beihang University,Beijing 100083,China [2]Department of Laboratory Medicine,State Key Laboratory of Biotherapy and Cancer Center,West China Hospital,Sichuan University and Collaborative Innovation Center,Chengdu 610041,China [3]Nano Biomedical Research Centre,School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200030,China [4]Wuhan Chain Medical Labs,Wuhan,Hubei 430011,China [5]Department of Clinical Laboratory,Zhongnan Hospital of Wuhan University,Wuhan,Hubei 430071,China [6]Department of Biomedical Engineering,City University of Hong Kong,China

出　　处：《Research》2021年第1期94-102,共9页研究（英文）

基　　金：supported by the Special Research Funding on COVID19 from Sichuan Province of China(Grant No.2020YFS0004);Science and Technology Department,Ministry of Education of the People’s Republic of China,the NSFC(No.32071407 and No.62003023);the 111 Project(No.B13003);Innovation Research Plan from the Shanghai Municipal Education Commission(ZXWF082101/056);Exploratory Project of Natural Science Foundation of Shanghai(19ZR1476000);Beijing Advanced Innovation Center for Biomedical Engineering at Beihang University。

摘　　要：Sensitive detection of SARS-CoV-2 is of great importance for inhibiting the current pandemic of COVID-19.Here,we report a simple yet efficient platform integrating a portable and low-cost custom-made detector and a novel microwell array biochip for rapid and accurate detection of SARS-CoV-2.The instrument exhibits expedited amplification speed that enables colorimetric read-out within 25 minutes.A polymeric chip with a laser-engraved microwell array was developed to process the reaction between the primers and the respiratory swab RNA extracts,based on reverse transcriptase loop-mediated isothermal amplification(RT-LAMP).To achieve clinically acceptable performance,we synthesized a group of six primers to identify the conserved regions of the ORF1ab gene of SARS-CoV-2.Clinical trials were conducted with 87 PCR-positive and 43 PCRnegative patient samples.The platform demonstrated both high sensitivity(95.40%)and high specificity(95.35%),showing potentials for rapid and user-friendly diagnosis of COVID-19 among many other infectious pathogens.

关 键 词：instrument diagnosis SPECIFICITY 

分 类 号：Q503[生物学—生物化学]