NLRP3基因rs10754558位点单核苷酸多态性与慢性阻塞性肺疾病的关联研究  

Analysis of the association between NLRP3 rs10754558 polymorphism and chronic obstructive pulmonary disease

作　　者：伍慧妍[1,2] 蔡洁琳 周泳琳 杨欣 彭亮[1,2] Wu Huiyan;Cai Jielin;Zhou Yonglin;Yang Xin;Peng Liang(The Fifth Affiliated Hospital of Guangzhou Medical University,Guangzhou Guangdong 510799,China;KingMed School of Laboratory Medicine,Guangzhou Guangdong 510180,China;Medical College of Jiaying University,Meizhou Guangdong 514031,China)

机构地区：[1]广州医科大学附属第五医院,广东广州510799 [2]广州医科大学金域检验学院,广东广州510180 [3]嘉应学院医学院,广东梅州514031

出　　处：《中华临床实验室管理电子杂志》2021年第4期222-226,230,共6页Chinese Journal of Clinical Laboratory Management(Electronic Edition）

基　　金：广东省医学科学技术研究基金项目(B2021440);广东省基础与应用基础研究基金自然科学基金(2021A1515011081)。

摘　　要：目的探讨NLRP3 3’端非编码基因rs10754558变异位点和慢性阻塞性肺疾病(COPD)之间的相关性,为探索COPD早期诊治的分子学标志提供线索。方法回顾性分析2020年8月至2021年6月,广州医科大学附属第五医院收治入院的COPD患者样本113例和健康对照组样本84例,利用高分辨熔解曲线(HRM)方法检测其核酸SNP分型,收集受试者的临床资料及炎症指标,同时对COPD患者进行口咽部致病菌检查。采用χ^(2)检验,t检验,logistic回归模型统计分析基因与疾病间的相关性。结果 NLRP3 3’端非编码基因rs10754558位点的基因型及等位基因频率在COPD稳定期组、COPD急性加重期组(AECOPD组)和健康对照组中差异均无统计学意义(χ^(2)=2.798,P=0.592;χ^(2)=0.536,P=0.765)。logistic回归分析显示,携带G等位基因与携带C等位基因型的患者未增加COPD的发生风险(P>0.05),但在炎症因子CRP的表达上,携带G等位基因的CRP水平显著高于携带C等位基因(P=0.016)。结论 COPD与NLRP3 rs10754558位点多态性无明显相关。COPD患者炎症因子表达升高,G等位基因可能与COPD患者炎症因子CRP的高表达有关。Objective To provide a potential molecular marker for the early diagnosis and treatment of COPD through investigating the correlation between rs10754558 mutation of NLRP3 3’non coding gene and chronic obstructive pulmonary disease(COPD).Methods A total of 113 COPD patients and 84 healthy controls were collected from August 2020 to June 2021 in the Fifth Affiliated Hosptial of Guangzhou Medical University.The SNP typing of nucleic acid was detected by HRM method.Meanwhile,clinical data and inflammatory indexes were collected,and pathogenic bacteria in the oropharynx were examined in COPD patients.The χ^(2) test,t test and logistic regression model were used to analyze the correlation between SNPs and COPD.Results There was no significant difference in genotype and allele frequency of NLRP3 rs10754558 among stable COPD group,AECOPD group and healthy control group(χ^(2)=2.798,P=0.592;χ^(2)=0.538,P=0.765).Logistic regression analysis showed that patients with G allele and C allele had no increased risk of COPD(P>0.05).However,the level of CRP in G allele was significantly higher than that in C allele(P=0.016).Conclusions There was no significant correlation between COPD and rs10754558 polymorphism of NLRP3.The expression of inflammatory factors was increased in COPD patients.G allele may be related to the high expression of CRP in patients with COPD.

关 键 词：慢性阻塞性肺疾病 NLRP3 多态性 

分 类 号：R563.9[医药卫生—呼吸系统]