茶轮斑病菌的生物学特性研究  

Biological Characteristics of Pestalotiopsi theae Steyaert

作　　者：谭荣荣[1] 毛迎新[1] 黄丹娟 王红娟[1] 陈勋[1] 王友平[2] TAN Rong-rong;MAO Ying-xin;HUANG Dan-juan;WANG Hong-juan;CHEN Xun;WANG You-ping(Institute of Fruit and Tea of Hubei Academy of Agricultural Sciences/Fruit and Tea Division of Hubei Innovation Center of Agricultural Science of Techonology,Wuhan,Hubei 430064,China;Institute of Plant Protection,Soil and Fertilizers of Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064,China)

机构地区：[1]湖北省农业科学院果树茶叶研究所/湖北省农业科技创新中心果茶分中心,湖北武汉430064 [2]湖北省农业科学院植保土肥研究所,湖北武汉430064

出　　处：《茶叶学报》2021年第4期198-204,共7页Acta Tea Sinica

基　　金：国家茶叶产业技术体系(CARS-19);湖北省农业科技创新中心资助项目(2016-620-000-001-032)。

摘　　要：采用生长速率法和产孢量的测定来研究茶轮斑病菌的生物学特性,为该类病害的田间科学防治提供理论依据。室内试验表明光照、温度、湿度、营养、酸碱度对茶轮斑病菌孢子和菌丝的生长有很重要的影响。不同光照条件下,菌丝均可生长,全光照条件下有最大产孢量。在22~30℃条件下,病原菌均可生长,最适合的生长温度是25~28℃。28℃时有最大产孢量,当温度升至32℃,产孢量为零。pH在4.5~8范围内茶轮斑病菌分生孢子均可萌发且芽管伸长较好,其中菌落生长的最适pH值为6.0,当pH为7时可达到最大产孢量。最适培养基为PDA培养基,且以蔗糖为碳源的培养基适宜菌丝的生长,以磷酸二氢铵为氮源的培养基菌丝生长速度快。Biological properties of Pestalotiopsis theae Steyaert were characterized by means of colony growth and spore production to aid the study on control of the ring spot disease on tea plants.The indoor experiments showed significant effects of light,temperature,humidity,nutrition,and pH on the growth of spores and hyphae of the pathogen.The hyphae could grow under varied light conditions,and the spore production maxed under full light exposure.The mycelium could develop in the range of 22~30℃and optimally at 25~28℃,while the maximum spore generation occurred at 28℃but ceased completely when temperature rose to 32℃.At pH 4.5~8,the conidia germinated with normal germ tube elongation.The optimum pH for the pathogen growth was 6.0,and for the peak spore production,pH 7.PDA was the most suitable culture medium for P.theae Steyaert.The mycelia grew best when sucrose was the carbon source and propagated most rapidly when ammonium dihydrogen phosphate was the nitrogen source.

关 键 词：茶轮斑病菌 生物学特性 致病表现 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]