小麦矮秆突变体je0098的遗传分析与其矮秆基因定位  

Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene

作　　者：付美玉 熊宏春 周春云 郭会君[2] 谢永盾[2] 赵林姝[2] 古佳玉[2] 赵世荣[2] 丁玉萍[2] 徐延浩 刘录祥[2] FU Mei-Yu;XIONG Hong-Chun;ZHOU Chun-Yun;GUO Hui-Jun;XIE Yong-Dun;ZHAO Lin-Shu;GU Jia-Yu;ZHAO Shi-Rong;DING Yu-Ping;XU Yan-Hao;LIU Lu-Xiang(Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/National Center of Space Mutagenesis for Crop Improvement,Beijing 100081,China)

机构地区：[1]长江大学农学院/主要粮食作物产业化湖北省协同创新中心,湖北荆州434025 [2]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/国家农作物航天诱变技术改良中心,北京100081

出　　处：《作物学报》2022年第3期580-589,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31801346);中国农业科学院基本科研业务费专项(Y2020YJ09);国家重点研发计划项目(2016YFD0102100)资助。

摘　　要：倒伏易引发小麦严重减产,发掘和利用优异矮秆基因是培育高产抗倒伏小麦新品种的关键。本研究以京411(WT)及其经EMS诱变获得的产量相关性状优良的矮秆突变体je0098为试验材料,对其株高进行遗传分析,结合外显子捕获测序和遗传连锁分析定位矮秆基因。3年田间株高数据统计分析表明,je0098与WT相比株高降低15 cm,组织细胞学观察结果显示,je0098与WT相比节间细胞长度缩短18%,暗示je0098的矮化是由于节间细胞长度变短所致;赤霉素敏感性分析表明,je0098为赤霉素敏感型矮秆突变体。利用WT和je0098杂交构建的由344个单株组成的F_(2)分离群体,结合F_(2):3家系表型数据,选取矮秆纯合和高秆单株构建混池,对两亲本和子代混池分别进行外显子捕获测序,在2D染色体上定位到一个具有降秆效应的数量性状位点(QTL)。结合全基因组重测序所得SNP位点,在2D染色体开发了6个KASP分子标记,对F_(2)单株进行基因分型。利用QTL IciMapping作图软件构建遗传连锁图谱,结合3年田间表型数据,将矮秆基因定位在20.77~28.84 Mb区间内,遗传距离为11.48 cM。本研究结果为突变体je0098矮秆基因的功能研究以及育种利用奠定了基础。Lodging easily causes severe decrease in wheat yields.Identification and utilization of favorable dwarfing genes is the key to develop new varieties with high yield and lodging resistance.In this study,a dwarf mutant je0098 as material was induced by EMS mutagenesis from Jing 411(WT)and had fine characteristics in yield components.We mapped the dwarfing gene through genetic analysis of plant height,and combining with exon capture sequencing and genetic linkage analysis.Statistical analyses of plant height in three-year field experiment suggested that plant height of je0098 was 15 cm lower than that of WT.Histocytological analysis of je0098 and WT indicated that the internode cell length of je0098 was about 18%shorter than that of WT,suggesting that the shorter internode cell length caused the dwarfism of je0098.Gibberellic acid treatment showed that je0098 was a gibberellic acid-sensitive dwarf mutant.An F_(2) segregation population consisting of 344 individuals was constructed by crossing WT and je0098.Combining with the phenotypic data of F_(2):3 families,dwarf homozygous and tall individuals were selected to construct progeny pools.Exon capture sequencing was performed on the two parents and progeny pools,respectively.A quantitative trait locus(QTL)with effects on reduced height was identified on chromosome 2D.Based on SNPs detected by genome-wide sequencing,six KASP markers were developed on chromosome 2D to genotype F_(2) individuals.Genetic linkage map was constructed using QTL IciMapping.Combining with phenotype data of three-year field experiment,the dwarfing gene was mapped in the range of 20.77-28.84 Mb with genetic distance of 11.48 cM.These results will lay the foundation for further func-tional research of je0098 and its application in wheat breeding.

关 键 词：小麦 株高 矮秆基因 BSA 分子标记 

分 类 号：S512.1[农业科学—作物学]