中国黄麻炭疽病病原菌的分离鉴定及系统发育分析  

Identification and phylogenetic analysis of the pathogen of jute anthracnose in China

作　　者：郭艳春 姚嘉瑜 张镕斌 陈思远 何青垚 陶爱芬 方平平[1,2] 祁建民 张列梅[1] 张立武 GUO Yan-Chun;YAO Jia-Yu;ZHANG Rong-Bin;CHEN Si-Yuan;HE Qing-Yao;TAO Ai-Fen;FANG Ping-Ping;QI Jian-Min;ZHANG Lie-Mei;ZHANG Li-Wu(Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops/Fujian Key Laboratory for Crop Breeding by Design/College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Experiment Station of Ministry of Agri-culture and Rural Affairs for Jute and Kenaf in Southeast China/Public Platform of Fujian for Germplasm Resources of Bast Fiber Crops/Fujian International Science and Technology Cooperation Base for Genetics,Breeding and Multiple Utilization Development of Southern Economic Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Center for Genomics and Biotechnology,Haixia Institute of Science and Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China.)

机构地区：[1]福建农林大学农学院/作物遗传育种与综合利用教育部重点实验室/福建省作物设计育种重点实验室,福建福州350002 [2]福建农林大学农业农村部东南黄红麻实验观测站/福建省麻类种质资源共享平台/福建省南方经济作物遗传育种与多用途开发国际科技合作基地,福建福州350002 [3]福建农林大学海峡联合研究院基因组与生物技术中心,福建福州350002

出　　处：《作物学报》2022年第3期770-777,I0001-I0006,共14页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31771369);国家现代农业产业技术体系建设专项(CARS-19-E06)资助。

摘　　要：炭疽病是严重影响黄麻纤维产量和品质的主要真菌性病害,明确炭疽病病原菌种类并测定其致病力有助于黄麻抗病育种和优异抗病基因资源挖掘。本研究从福建省福州市、福建省漳州市、河南省信阳市、安徽省六安市、浙江省杭州市、广西南宁市和湖南省长沙市等7个黄麻生产区采集黄麻炭疽病病原菌样本,分离纯化出92个菌株;rDNA-ITS区域的序列分析表明,其中11个为典型炭疽病病原菌。LSU区域的系统进化树和形态学特征鉴定显示,ZZ4、GX19等10个菌株为胶胞炭疽菌(Colletotrichum gloeosporioides),菌株CS3为黑线炭疽菌(Colletotrichum dematium)。人工接种的致病力测定表明,不同炭疽菌菌株致病力存在显著差异,其中胶胞炭疽菌菌株GX19致病力最强,表现为优势菌株。这些结果为黄麻炭疽病抗性基因位点挖掘和有效防治奠定了基础。Anthracnose is a major fungal disease that seriously affects the yield and quality in jute fiber.Identifying the types of anthracnose pathogens and determining their pathogenicity will help jute disease-resistant breeding and develop elite dis-ease-resistant gene resources.The 92 strains were isolated and purified from samples of diseased jute,which were collected from 7 jute production areas including Fuzhou and Zhangzhou in Fujian Province,Xinyang City in Henan Province,Liu’an City in Anhui Province,Hangzhou City in Zhejiang Province,Nanning City in Guangxi Province,and Changsha City in Hunan Province.The sequence analysis of the rDNA-ITS region showed that 11 out of the 92 strains were typical anthracnose pathogens.Further identification of the phylogenetic tree of the LSU region and morphological characteristics revealed that 10 strains,including ZZ4 and GX19,were Colletotrichum gloeosporioides,while the strain of CS3 was Colletotrichum dematium.The pathogenicity test of artificial inoculation indicated that there was a significant difference in pathogenicity between Colletotrichum gloeosporioides and Colletotrichum dematium.These results laid a basis for identification of resistance gene loci and effective controlling of anthracnose in jute.

关 键 词：黄麻 炭疽菌 致病力测定 系统发育 

分 类 号：S435.63[农业科学—农业昆虫与害虫防治]