DNAzyme as a rising gene-silencing agent in theranostic settings  

作　　者：Nan Zhang 

机构地区：[1]Neuroscience Program,Department of Neurology,Houston Methodist Research Institute,Houston,TX,USA

出　　处：《Neural Regeneration Research》2022年第9期1989-1990,共2页中国神经再生研究（英文版）

基　　金：supported by a National Ataxia Foundation Young Investigator-SCA Award,No.93000(to NZ).

摘　　要：Decades of biochemical studies have advanced DNA beyond its primary role as genetic blueprint.DNAzymes are single-stranded enzymatic DNA molecules that do not exist in nature.They are ideal candidates for gene silencing owing to their scalability by solid-phase synthesis(without batch variations),reprogrammability by directed evolution and local sequence alterations,compatibility with diverse delivery methods,and capability of achieving high catalytic turnover independent of any auxiliary proteins.With these unique features,various artificially evolved DNAzymes have been employed as theranostic tools in designing biosensors and logic gates,RNA/DNA cleavage and ligation,phosphorylation and dephosphorylation,DNA photorepair,and peptide side-chain modifications,to name but a few(Ponce-Salvatierra et al.,2021).This perspective will focus on the functional aspects and therapeutic potentials of RNA-cleaving DNAzymes.

关 键 词：compatibility auxiliary BATCH 

分 类 号：R450[医药卫生—治疗学]