HPLC⁃UV⁃ELSD法同时测定防风通圣丸中8种成分  

Simultaneous determination of eight constituents in Fangfeng Tongsheng Pills by HPLC

作　　者：赵迪[1] 刘学芳[1] 董浩然[1] 冯素香[1] 李璐睿惠 ZHAO Di;LIU Xue-fang;DONG Hao-ran;FENG Su-xiang;LI Lu-rui-hui(Academy of Chinese Medical Sciences,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学中医药科学院,河南郑州450046

出　　处：《中成药》2022年第1期38-41,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(81773892);河南中医药大学科研苗圃工程项目(MP2020⁃68)。

摘　　要：目的建立HPLC法同时测定防风通圣丸(川芎、桔梗、甘草等)中川芎嗪、栀子苷、升麻素苷、甘草苷、5⁃O⁃甲基维斯阿米醇苷、黄芩苷、桔梗皂苷D、黄芩素的含量。方法该药物80%甲醇提取液的分析采用Venusil MP C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相乙腈⁃0.1%醋酸,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长260 nm;ELSD漂移管温度90℃;雾化气体积流量2.5 mL/min;雾化温度36℃。结果8种成分在各自范围内线性关系良好(r≥0.9995),平均加样回收率99.35%~99.60%,RSD 0.52%~1.31%。结论该方法准确灵敏,重复性好,可用于防风通圣丸的质量控制。AIM To establish an HPLC method for the simultaneous content determination of ligustrazine,gardenoside,prim⁃O⁃glucosylcimifugin,glycyrrhizin,5⁃O⁃methylvisammioside,baicalin,platycodin D and baicalein in Fangfeng Tongsheng Pills(Chuanxiong Rhizoma,Platycodonis Radix,Glycyrrhizae Radix et Rhizoma,etc.).METHODS The analysis of 80%methanol extract of this drug was performed on a 25℃thermostatic Venusil MP C_(18) column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile⁃0.1%acetic acid flowing at 1.0 mL/min in a gradient elution manner,the detection wavelength was set at 260 nm,the drift tube temperature of ELSD was 90℃,the volumetric flow rate of atomizing airflow was 2.5 mL/min,and the atomizing temperature was 36℃.RESULTS Eight constituents showed good linear relationships within their own ranges(r≥0.9995),whose average recoveries were 99.35%-99.60%with the RSDs of 0.52%-1.31%.CONCLUSION This accurate,sensitive and reproducible method can be used for the quality control of Fangfeng Tongsheng Pills.

关 键 词：防风通圣丸 化学成分 HPLC⁃UV⁃ELSD 

分 类 号：R927.2[医药卫生—药学]