右美托咪定不同时间点干预心肌缺血再灌注损伤中JAK-STAT3作用分析  

Effect of Dexmedetomidine on JAK-STAT3 in Myocardial Ischemia-Reperfusion Injury at Different Time Points.

作　　者：陈朋 袁素平 王亚娟[3] 宫伟 黄珊 Chen Peng;Yuan Suping;Wang Yajuan(Department of Anesthesiology,Second Hospital of Baoding,Baoding,Hebei 071000;Department of Anesthesiology,Chinese People′s Liberation Army No.82 Army Group Military Hospital,Baoding,Hebei 071000;Department of Anesthesiology,Baoding Maternal and Child Health Hospital,Baoding,Hebei 071000,China.)

机构地区：[1]保定市第二医院麻醉科,河北保定071000 [2]中国人民解放军陆军第八十二集团军医麻醉科,河北保定071000 [3]保定市妇幼保健院麻醉科,河北保定071000 [4]保定市第一医院麻醉科,河北保定071000

出　　处：《四川医学》2021年第12期1198-1204,共7页Sichuan Medical Journal

摘　　要：目的分析右美托咪定不同时间点干预心肌缺血再灌注损伤中的JAK-STAT3作用。方法实验动物选择自发性高血压清洁级雄性大鼠100只,将以上大鼠按照随机原则分为5组,每组大鼠20只,分别为假手术组、模型对照组、前处理组、后处理组、前处理联合后处理组,分析五组大鼠心电图ST段改变、心肌凋亡以及CK-MB、LDH、MDA、TNF-a、IL-1β、IL-6、JAK mRNA、p-STAT3 mRNA、JAK、p-STAT3、Caspase-3水平差异。结果前处理以及后处理联合组的ST的抬高情况显著低于前处理组以及后处理组,前处理以及后处理联合组的心肌细胞梗死面积、危险区域面积、心肌细胞凋亡率显著低于前处理组以及后处理组(P<0.05);前处理以及后处理联合组的CK-MB、LDH、MDA、TNF-a、IL-1β、IL-6、JAK mRNA、p-STAT3 mRNA、JAK1、p-STAT3、Caspase-3显著低于前处理组以及后处理组,GSH-Px显著高于前处理组以及后处理组(P<0.05)。结论右美托咪定前处理或后处理均可抑制心肌缺血再灌注损伤大鼠JAK-STAT3通路,抑制炎症因子表达,减轻氧化应激反应,保护心肌细胞和心功能,但前处理联合后处理的效果优于单独的前处理或后处理,临床应依据具体情况进行选择。Objective To analyze the role of JAK-STAT3 in myocardial ischemia-reperfusion injury intervened by dexmedetomidine at different time points.Methods 100 male spontaneously hypertensive rats of clean grade.were randomly divided into 5 groups,20 rats per group,including sham operation group,model control group,pretreatment group,post-treatment group and pretreatment combined with postconditioning group.The levels of LDH,MDA,TNF-a,IL-1β,IL-6,JAK mRNA,p-STAT3 mRNA,JAK,p-STAT3 and caspase-3 levels were analyzed.Results The elevation of ST in the pretreatment combined with postconditioning group was significantly lower than that in either pretreatment group or the postconditioning group;infarct area,area of dangerous zone and apoptosis rate of myocardial cells in the pretreatment and postconditioning combined group were significantly lower than those in either the pretreatment group or the postconditioning group(P<0.05);CK-MB,LDH,MDA,TNF-a,IL-1β,IL-6,JAK mRNA,p-STAT3 mRNA,JAK1,p-STAT3,Caspase-3 in the pretreatment and postconditioning combined group were significantly lower than those in either the pretreatment group or the postconditioning group(P<0.05),while GSH Px in the pretreatment and postconditioning combined group was significantly higher than that in either the pretreatment group or the post-treatment group(P<0.05).Conclusion Dexmedetomidine pre-treatment or post-treatment can inhibit the JAK-STAT3 pathway in rats with myocardial ischemia-reperfusion injury,decrease the expression of inflammatory factors,reduce oxidative stress,and protect myocardial cells and cardiac function.The effect by pre-treatment combined with post-treatment is better than that of pre-treatment or post-treatment alone,and the clinical choice should be based on specific conditions.

关 键 词：右美托咪定 心肌细胞损伤 心肌缺血再灌注 JAK1 STAT3 

分 类 号：R-332[医药卫生]