生物有机肥DNBF32对黑土中阿特拉津消减及土壤细菌群落结构的影响  

Effects of bio-organic fertilizer DNBF32 on removal of atrazine and bacterial communities in black soil

作　　者：姜昭[1] 马月璇[1] 闫轶文 王建民 刘畅[1] 佟姗姗 张智伟 JIANG Zhao;MA Yuexuan;YAN Yiwen;WANG Jianmin;LIU Chang;TONG Shanshan;ZHANG Zhiwei(School of Resources and Environment,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《东北农业大学学报》2022年第2期13-19,共7页Journal of Northeast Agricultural University

基　　金：国家自然科学基金面上项目(31972941)。

摘　　要：试验研究生物有机肥DNBF32对除草剂阿特拉津在玉米田土壤中残留量及玉米生长情况的调控作用,并通过关注土壤细菌群落结构对DNBF32施用响应探讨DNBF32强化阿特拉津消减及调节玉米生长潜在作用机制。田间试验结果表明,DNBF32施用量为150 kg·hm^(-2)以上时,玉米成熟期土壤中无阿特拉津检出。上述施用水平的DNBF32可使玉米株高和产量较对照处理相比分别提升7.64%和8.30%。上述结果说明DNBF32具有强化土壤中残留阿特拉津消减及调节玉米生长的能力。此外,当DNBF32施用量为0~225 kg·hm^(-2)时,玉米成熟期农田土壤中可培养细菌菌落总数随DNBF32施用量增加而显著提升,各生物有机肥施用处理与未施肥对照处理相比均达到显著差异水平(P<0.05)。高通量测序结果表明,施用量为150 kg·hm^(-2)的DNBF32在提升土壤细菌多样性同时,可调控土壤出现具有污染物降解、光合能力等生态功能的微生物。结合相关性分析结果表明,DNBF32施用可通过调节土壤细菌数量及群落结构强化阿特拉津消减。研究为生物有机肥在黑土污染修复及农业环境保护中应用提供方法借鉴。In this study,the abilities of bio-organic fertilizer DNBF32 to remove atrazine in black soil of corn field and corn growth regulation were investigated,meanwhile,the response of the soil bacterial community structure to various amounts of applied DNBF32 were also reported.The results of field experiment showed that there was no atrazine could be detected in treatments that applied more than 150 kg·hm^(-2) of DNBF32,and the plant height and maize yield in treatment that appling 150 kg·hm^(-2) of DNBF32 was enhanced 7.64%and 8.30%by comparing with that of without DNBF32 adding treatment.These results suggested that the applied DNBF32 could enhance the decreasing rate of atrazine which residue in soil as well as increase maize plant height and maize yield.In addition,the number of culturable bacteria in the field soil during maize harvest period was positively correlated with the application amount of DNBF32(0 to 225 kg·hm^(-2))and the difference among the treatments were statistically significant. High throughput sequencing results showed that 150 kg · hm^(-2) of DNBF32 increased soil bacterial diversity, and some microorganisms with special ecological functions, such as pollutant degradation and photosynthetic capacity, were detected in soil. Furthermore, correlation analysis suggests that DNBF32 could enhance atrazine removal by regulating culturable bacteria number and bacterial diversity. These results could provide new insights into the application of bio organic fertilizer in the field of the black soil remediation and agriculrutal environment protection.

关 键 词：生物有机肥 玉米 黑土 阿特拉津 细菌群落结构 

分 类 号：S155.27[农业科学—土壤学] S154.3[农业科学—农业基础科学]