黄秋葵多糖对耐力训练小鼠自由基代谢和糖代谢影响的研究  

Effect of Okra Polysaharide on Free Radical Metabolism and Glycometabolism in Endurance Training Mice

作　　者：詹晓东 梁益军 ZHAN Xiaodong;LIANG Yijun(Basic Teaching Department,Beihai Campus of Guilin University of Electronic Science and Technology,Beihai 536000,China)

机构地区：[1]桂林电子科技大学北海校区基础教学部,广西北海536000

出　　处：《云南农业大学学报（自然科学版）》2022年第1期105-110,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：新世纪广西高等教育教学改革工程项目(2014JGB140)。

摘　　要：【目的】研究黄秋葵多糖对耐力训练小鼠自由基代谢和糖代谢的影响。【方法】选取昆明种健康雄性小鼠为试验对象,以负荷游泳训练方式建立耐力运动模型。将试验小鼠随机分为安静组(A组)、对照组(B组)和黄秋葵多糖组(低、中、高剂量组分别为C_(1)、C_(2)、C_(3)组)进行试验小鼠活动状态行为学观察和体质量测定,并测定肌酸激酶(CK)、血清乳酸脱氢酶(LDH)、血乳酸(BL)、血尿素氮(BUN)、谷丙转氨酶(GPT)和谷草转氨酶(GOT)、肌糖原和肝糖原含量以及组织抗氧化指标超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性和丙二醛(MDA)含量。【结果】与A组相比,B组体质量增长缓慢,小鼠疲劳特征明显,游泳至力竭时间短,CK、LDH、BL、BUN、GPT和GOT含量升高,肌糖原和肝糖原含量无显著变化,肝脏SOD活性升高,肝脏和骨骼肌CAT活性降低,肝脏和骨骼肌MDA含量升高。C_(3)组(400 mg/kg剂量)黄秋葵多糖耐力训练小鼠体质量增加,行为学特征改善,游泳至力竭时间延长,CK、LDH、BL、BUN、GPT和GOT含量降低,肌糖原和肝糖原含量升高,肝脏SOD活性降低,肝脏和骨骼肌CAT活性升高,肝脏和骨骼肌MDA含量降低。【结论】黄秋葵多糖有助于改善耐力训练小鼠自由基代谢和糖代谢,从而提高小鼠运动能力,发挥抗疲劳功能。[Purpose]To study the effects of okra polysaharide on free radical metabolism and glucose metabolism in endurance training mice.[Methods]Healthy Kunming male mice were selected as the experimental objects,and the endurance exercise model was established by load swimming training.The mice were randomly divided into quiet group(group A),control group(group B),and okra polysaharide group(group C,low,moderate and high dose group named C_(1),C_(2) and C_(3)).The activity,behavior and body weight of the mice were observed.The contents of creatine kinase(CK),lactate dehydrogenase(LDH),blood lactic acid(BL),blood urea nitrogen(BUN),glutamic pyruvic transaminase(GPT)and glutamic oxaloacetic transaminase(GOT)in serum,muscle glycogen and liver glycogen,and the activities of superoxide dismutase(SOD)and catalase(CAT)while the content of malondialdehyde(MDA)in tissues were determined.[Results]Compared with group A,the weight of mice in group B increased slowly,the fatigue characteristics of mice were obvious,the time from swimming to exhaustion was short,the contents of CK,LDH,BL,BUN,GPT and GOT increased,the contents of muscle glycogen and liver glycogen had no significant change,the activity of SOD in liver increased,the activity of CAT in liver and skeletal muscle decreased,and the content of MDA in liver and skeletal muscle increased.In group C_(3),after endurance training with 400 mg/kg of okra polysaharide,the mice gained weight,improved behavioral characteristics,prolonged the time from swimming to exhaustion,decreased the contents of CK,LDH,BL,BUN,GPT and GOT,increased the content of muscle glycogen and liver glycogen,decreased the activity of SOD in liver,increased the activity of CAT in liver and skeletal muscle,and decreased the content of MDA in liver and skeletal muscle.[Conclusion]Okra polysaharide can improve the free radical metabolism and glucose metabolism of endurance training mice,so as to improve the exercise ability of mice and exert the anti fatigue function.

关 键 词：黄秋葵多糖 自由基代谢 糖代谢 抗疲劳功能 耐力训练小鼠 

分 类 号：R285.5[医药卫生—中药学]