女黄扶正发酵物对脾虚大鼠肠道结构及免疫的影响  

Effects of Fermented Nyuhuang Fuzheng on the Intestinal Structure and Immunity of Rats with Spleen Deficiency

作　　者：闫志强[1] 翟少钦[1] 张邑帆[1] 陈春林[1] YAN Zhiqiang;ZHAI Shaoqin;ZHANG Yifan;CHEN Chunlin(Chongqing Academy of Animal Sciences,Chongqing 402460,China)

机构地区：[1]重庆市畜牧科学院,重庆402460

出　　处：《云南农业大学学报（自然科学版）》2022年第1期120-125,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：重庆市自然科学基金项目(cstc2019jcyj-msxmX0382)。

摘　　要：【目的】研究女黄扶正发酵物对脾虚大鼠肠道结构和免疫的影响。【方法】将80只SPF级SD健康大鼠随机分为空白组、模型组、发酵菌组和女黄扶正发酵物组,除空白组外,其余处理采用饮食失节加苦寒泻下法人工复制脾虚模型;模型复制成功后,发酵菌组和女黄扶正发酵物组分别饲喂添加发酵菌和女黄扶正发酵物的饲料,空白组和模型组饲喂实验鼠维持饲料。试验结束时,各组大鼠称量并测定血常规和免疫器官指数;观察大鼠肠道组织形态变化,测定绒毛高度、绒毛宽度和隐窝深度;ELISA法检测大鼠血清IL-2、IL-10、TNF-α和IFN-γ以及肠组织sIgA水平。【结果】女黄扶正发酵物组大鼠血液白细胞数和免疫器官指数升高,与模型组相比差异显著(P<0.05);其十二指肠和回肠绒毛高度增加,与模型组相比差异极显著(P<0.01);其血清IFN-γ、TNF-α和IL-10以及肠黏膜组织sIgA含量增加,与模型组相比差异极显著(P<0.01)。【结论】女黄扶正发酵物可通过上调免疫器官指数、IL-2、IL-10、TNF-α、IFN-γ及肠黏膜sIgA水平,进而调节脾虚大鼠的免疫功能,并改善和修复脾虚大鼠受损肠黏膜,恢复肠道黏膜免疫。[Purpose]To study the effect of fermented Nyuhuang Fuzheng on the intestinal structure and intestinal immunity in rats with spleen deficiency.[Methods]Eighty SPF-level healthy SD rats were randomly divided into blank group,model group,fermented bacteria group and fermented Nyuhuang Fuzheng group.Except the blank group,the other groups were artificially replicated spleen-deficiency model by the method of eating disorder and bitter cold purging.After the model replicated successfully,the fermented bacteria group and the fermented Nyuhuang Fuzheng group were fed with fermented bacteria or fermented Nyuhuang Fuzheng,and the blank group and the model group were fed with experimental mice maintenance feed.At the end of the experiment,the rats in each group were weighed,the blood routine and the immune organ index were measured;the morphological changes of the rat’s intestinal tissues were observed,the height of villi,the width of villi and the depth of crypts were measured,and the serum IL-2,IL-10,TNF-α,IFN-γand intestinal tissue sIgA levels of rats were detected by ELISA.[Results]Compared with the model group,the num-ber of white blood cell and immune organ index increased in the fermented Nyuhuang Fuzheng group of rats,the difference was significant(P<0.05);the height of duodenal and ileum villi in the fermen-ted Nyuhuang Fuzheng group was increased,and compared with the model group,the difference was extremely significant(P<0.01);the serum IFN-γ,TNF-α,IL-10 and intestinal mucosal tissue sIgA levels in the fermented Nyuhuang Fuzheng group increased,and the difference was extremely signi-ficant compared with the model group(P<0.01).[Conclusion]The fermented Nyuhuang Fuzheng can regulate the immune function of rats with spleen deficiency by up-regulating the index of im-mune organ index,IL-2,IL-10,TNF-α,IFN-γ,and intestinal mucosal sIgA levels,and improve and repair the immune function of rats with spleen deficiency,recovering the intestinal mucosa immunity.

关 键 词：女黄扶正发酵物 脾虚 大鼠 肠道结构 肠道免疫 

分 类 号：S853.73[农业科学—临床兽医学]