3种因素对木棉组培苗生根的影响  

Effects of Three Factors on the Rooting of Tissue Culture Seedling of Bombax ceiba

作　　者：孙大宽 马莉 杨宏艳[1] 马焕成[1] 郑晟靖 唐军荣[1] SUN Dakuan;MA Li;YANG Hongyan;MA Huancheng;ZHENG Chengjing;TANG Junrong(Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China,Southwest Forestry University,Kunming 650224,China;Forest and Grassland Resource Management Station of Honghe Prefecture,Mengzi 661199,China)

机构地区：[1]西南林业大学,西南地区生物多样性保育国家林业和草原局重点实验室,云南昆明650224 [2]红河州森林和草原资源管理站,云南蒙自661199

出　　处：《云南农业大学学报（自然科学版）》2022年第1期137-144,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：西南林业大学木棉纤维人工林产业化培育省创新团队(2018CH014);国家自然科学基金项目(31560207,31260175);云南省“云岭教学名师”培育项目。

摘　　要：【目的】进一步完善木棉的组培快繁体系,实现木棉组培苗不定根的定向诱导,提高木棉生根苗的移栽成活率。【方法】以木棉组培丛生芽为材料,通过激素、纵向划痕和暗培养等3种处理促进根系诱导,比较不同处理下木棉组培苗的生根率、根系数量和移栽成活率,同时对生根过程进行显微观察。【结果】在单独添加NAA和IBA时,最高生根率分别为19.66%和39.15%;而NAA与IBA组合处理的生根率可达95.80%,优于两者单独添加处理,且生根苗以1条不定根为主。纵向划痕处理以2~3个划痕效果较好,其生根率可达94%以上,生根苗以2条不定根为主。暗培养处理以6 d最佳,生根率为97.36%,生根苗以1条不定根为主。显微观察发现:木棉组培苗生根属于皮层生根类型。对1、2和3条不定根等3种类型的生根苗进行移栽,其移栽成活率之间无显著差异(P>0.05),但生物量存在显著差异(P<0.05),其中3条不定根的移栽苗生物量最大。【结论】本研究优化了木棉组培苗的生根条件,为木棉组培苗的移栽炼苗以及不定根的定向调控提供了理论参考。[Purpose]To further improve the tissue culture and rapid propagation system of Bombax ceiba,realizing the directional induction of adventitious roots of tissue culture seedlings of B.ceiba,and improving the transplanting survival rate of root seedlings of B.ceiba.[Methods]The clustered buds of B.ceiba get from the tissue culture were used as materials.The root induction was promoted by hormone,longitudinal scratch and dark culture treatments,so as to compare the differences in the rooting rate,root number of tissue culture seedlings and transplanting survival rate under different treatments.The rooting process was observed by microscope.[Results]The highest root-ing rate was 19.66%and 39.15%respectively when NAA or IBA was added separately,and the root-ing rate was much higher when NAA and IBA used together,and the highest rooting rate was 95.80%,and the rooting seedling with one adventitious root accounted for the majority.In the treatment of lon-gitudinal scratches,the effect of two or three scratches was better,and the rooting rate could reach more than 94%,with two adventitious roots as the majority.In dark culture,the best treatment was six days,and the rooting rate was 97.36%,and the rooting seedling with one adventitious root accounted for the majority.The microscopic observation showed that the rooting of tissue culture seedling of B.ceiba belonged to species that generate roots form cortex.In addition,there was no significant differ-ence in transplanting survival rate among one,two or three adventitious of rooting seedling(P>0.05),but there was significant difference in biomass(P<0.05).Among them,the biomass of the trans-planted seedling with three adventitious roots was the largest.[Conclusion]In this study,the root-ing conditions of tissue culture seedling of B.ceiba were optimized,which provided a theoretical ref-erence for the transplanting of tissue culture seedling of B.ceiba and the directional regulation of ad-ventitious roots.

关 键 词：木棉 组培苗生根 生根率 不定根数量 

分 类 号：S723.132.6[农业科学—林木遗传育种]