外源Fe^(2+)对梨黄化叶光合能力及糖合成代谢的影响  

Effects of ferrous sulfate applied by foliar spray on photosynthesis and photoproducts metabolism of the chlorotic pear leaves(Pyrus bretschneideri‘Dangshan Suli’)

作　　者：贾兵[1] 董炜昱 郭国凌 张舒琴 陈猛 许波 衡伟[1] JIA Bing;DONG Weiyu;GUO Guoling;ZHANG Shuqin;CHEN Meng;XU Bo;HENGWei(School of Horticulture,Anhui Agricultural University,Hefei 230036,Anhui,China)

机构地区：[1]安徽农业大学园艺学院,安徽合肥230036

出　　处：《果树学报》2022年第1期22-35,共14页Journal of Fruit Science

基　　金：国家现代农业产业技术体系建设专项(CARS-29-14)。

摘　　要：【目的】探讨外源FeSO_(4)对梨黄化叶叶绿素含量、光合特性及其糖代谢的影响。【方法】以砀山酥梨正常和黄化植株为试材,对生长期黄化植株喷施0.2%FeSO_(4)溶液,测定叶片总叶绿素(Chl)、山梨醇、果糖、葡萄糖及蔗糖含量;分析了光合、糖代谢相关基因的表达。【结果】FeSO_(4)处理诱导了黄化叶内Chl合成,增强了其叶片净光合速率P_(n)、蒸腾速率T_(r)和叶绿素荧光参数F_(m)、F_(v)/F_(m),促进了叶内果糖、山梨醇和葡萄糖积累。FeSO_(4)处理后3、6、9、12 d,光合基因PSI subunit II、PSII psbD、Rubisco activase V和Chl a–b P4表达量均显著升高,山梨醇代谢相关基因S6PDH1、SDH2/3和其转运基因SOT8/14/15/26/30表达量则均在处理后6 d和9 d显著增加。而蔗糖合成基因SPS1/3和转运基因SUT1/2/3、TMT2表达量均在处理后3、6、9 d显著减少,而显著下降的SUS1/3/7/12则可能催化了蔗糖的降解。【结论】外源FeSO_(4)促进了黄化叶内Chl合成,调控了光合基因的表达,并显著增强了其光合作用,同时调控糖代谢相关基因,加速其合成、分解与转运,显著增加了叶内果糖和葡萄糖含量。【Objective】Dangshan Suli pear(Pyrus bretschneideri Rehd.)is extensively cultivated in the region of the Old Yellow River Valley,Anhui province,China.Currently,the iron-deficiency chlorosis has distributed widely and seriously restricted the development of pear industry in the region.It was shown in our previous work that FeSO_(4) applied by foliar spray could induce the regreening of chlorotic leaves in pears.However,little is known about the effects on the photosynthesis and the anabolism of its assimilated products.【Methods】The plants with normal and chlorotic leaves of Dangshan Suli pears(C_(N) and C_(C))were taken as test materials in the region of the Old Yellow River Valley,Dangshan county,Anhui province,China in May of 2020.The water solution of 0.2%FeSO_(4) was sprayed to running off using a powered backpack sprayer during the growth period with three replicates,one non-sprayed tree was left as a buffer between the treated trees and distilled water was used as control.All the trees used in this study were relatively consistent in tree shape and vigor,spaced at 4 m in row and 6 m between rows,without crops between the rows,trained with the open center system,and the orchard was managed as usual beside FeSO_(4) spray.The leave samples of C_(N) and C_(C) were taken at 3,6,9,and 12 days after FeSO_(4) application(T_(3 d),T_(6 d),T_(9 d) and T_(12 d)),respectively.The samples were brought back to the laboratory,and were quickly frozen in liquid nitrogen,and were stored in the refrigerator at-80℃till use.The content of total chlorophyll in the leaves was determined by 80%acetone extraction,and the chlorophyll fluorescence parameters were measured using portable pulse modulated fluorescence instrument(FMS-2).The photosynthetic characteristics of the leaves were detected using portable photosynthesis system(Li-6400,USA).The determination of sorbitol,sucrose,fructose and glucose contents in the leaves were conducted using High Performance Liquid Chromatoghraphy.The total RNA of the leave samples was extr

关 键 词：砀山酥梨 黄化叶 FeSO_(4) 光合特性 糖合成代谢 

分 类 号：S661.2[农业科学—果树学]