海南省火龙果溃疡病菌对吡唑醚菌酯敏感性分析  

Sensitivity analysis of pitaya canker disease Neoscytalidium dimidiatum to pyraclostrobin in Hainan province,China

作　　者：李希 汤敬诚 谢昌平[1] 仇芳 李晶[1] 李睿 王佳楠[1] LI Xi;TANG Jingcheng;XIE Changping;QIU Fang;LI Jing;LI Rui;WANG Jianan(College of Plant Protection,Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests,Ministry of Education,Haikou 570228,Hainan,China)

机构地区：[1]海南大学植物保护学院·热带农林生物灾害绿色防控教育部重点实验室,海口570228

出　　处：《果树学报》2022年第1期104-111,共8页Journal of Fruit Science

基　　金：海南大学高层次人才科研启动基金项目(KYQD(ZR)20028);海南省普通高等学校研究生创新科研课题(Hys2020-260)。

摘　　要：【目的】监测分析海南省火龙果溃疡病菌对吡唑醚菌酯的敏感基线及敏感性水平。【方法】采用菌丝生长速率法测定吡唑醚菌酯对海南省8个火龙果种植市(县)采集到的59株火龙果溃疡病菌的EC_(50)值,采用R语言进行数据分析。【结果】吡唑醚菌酯对供试菌株的EC_(50)范围为0.2540~2.2233μg·mL^(-1),EC_(50)均值为0.9841μg·mL^(-1);以58株菌株EC_(50)连续性正态分布时的均值0.9627μg·mL^(-1)作为海南省火龙果溃疡病菌对吡唑醚菌酯的敏感基线,海南省火龙果溃疡病菌均对吡唑醚菌酯表现为敏感;除乐东和白沙地区间敏感性差异显著外,其他地区间敏感性差异均不显著。【结论】海南省火龙果溃疡病菌对吡唑醚菌酯的敏感性差异较小,对吡唑醚菌酯仍较为敏感。【Objective】Pitaya canker disease caused by Neoscytalidium dimidiatum is the main disease of pitaya in Hainan province.With the expansion of pitaya planting area and the increase of planting years,the occurrence of N.dimidiatum in the planting area has been increasing.The pathogen mainly damages the stem and the fruit of pitaya and causes great loss in production.According to investigation,pyraclostrobin is used to control the disease in Hainan province all year round,but the sensitivity of N.dimidiatum to pyraclostrobin has not been reported yet.Therefore,the purpose of this study was to determine the sensitivity baseline and sensitivity level of N.dimidiatum to pyrazolesterim,so as to provide a reference for scientific application of N.dimidiatum in Hainan province.【Methods】N.dimidiatum collected from eight regions in Hainan province were isolated from the infected tissues.First,some diseased tissue pieces(5 mm×5 mm)were cut off and sterilized with 75%ethanol for 35 s and then with 5%NaClO for 40 s,and washed with sterile water for 3 times.The diseased tissue pieces were then pasted on PDA plates and cultured in an incubator at 28℃for 2-3 days.After mycelia grew on the PDA plate,a single mycelium was transferred to a new PDA plate and cultured for 5 days.The pathogen was preliminarily confirmed by microscopy after sporulating.Next,single spores isolated was used to obtain pure colonies.Finally,a total of 59 purified isolates were obtained and their sensitivities to pyraclostrobin were evaluated through mycelial growth rate method.The medium with final concentrations of 6,4,2,0.5 and 0.2μg·mL^(-1) were prepared for each isolate.The pyraclostrobin-free PDA medium was added with 1 mL acetone as the solvent control,and the PDA medium with 1 mL sterile water as the blank control.In addition,salicylhydroxamic acid with a final concentration of 50 mg·L^(-1) was added to all the tested mediums to reduce the effect of other respiratory pathway on the fungicide.The agar disks containing mycelium of N.dimidia

关 键 词：火龙果 溃疡病 吡唑醚菌酯 敏感性 

分 类 号：S667.9[农业科学—果树学] S436.679[农业科学—园艺学]