香蕉内生贝莱斯芽孢杆菌YX-11的分离鉴定与功能研究  

Isolation,Identification and Functional Properties of Endophytic Bacillus velezensis YX-11 from Banana

作　　者：李万芹 何鹏飞[1] 吴毅歆[1] 何鹏搏 Shahzad Munir 孔宝华[1] 李兴玉[1] 何月秋[1] LI Wan-qin;HE Peng-fei;WU Yi-xin;HE Peng-bo;Shahzad Munir;KONG Bao-hua;LI Xing-yu;HE Yue-qiu(Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学,云南昆明650201

出　　处：《江西农业学报》2021年第12期8-13,共6页Acta Agriculturae Jiangxi

基　　金：科技部重点研发计划项目“热带果树化肥农药减施增效技术集成研究与示范”(2017YFD0202100)。

摘　　要：为了找到安全有效的防控香蕉枯萎病的生防菌株,采用组织分离法从香蕉叶片中分离出内生细菌菌株YX-11。采用形态学、生物化学和16S rDNA基因序列分析,该菌株被鉴定为贝莱斯芽孢杆菌Bacillus velezensis。YX-11具有溶磷、解钾和固氮的能力,还可以促进香蕉的生长,能抑制10种植物病原菌,其中对香蕉枯萎病菌、烟草疫霉病菌和烟草赤星病菌的抑制率分别达71.30%、72.77%和72.19%。它的带菌苗结合生物有机肥施用,以及多次喷施对田间香蕉枯萎病的防治效果达75.56%。In order to find a safe and effective strain for biocontrol of banana Fusarium wilt,the endophytic strain YX-11 was isolated from banana leaf by tissue isolation method.The strain was identified as Bacillus velezensis based on morphology,biochemistry and 16S rDNA gene sequence.It has the ability to dissolve phosphorus and potassium,fix nitrogen and promote banana growth.It can inhibit 10 important pathogens,among which the inhibition rates of Fusarium oxysprum f.sp.cubense,Phytophthora parasitica and Alteraria alternata were 71.30%,72.77%and 72.19%respectively.When it was combined with organic fertilizer and sprayed repeatedly in the field,the control effect of banana Fusarium wilt was 75.56%.

关 键 词：内生菌 香蕉 枯萎病菌 分离鉴定 防治效果 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]