葡萄籽原花青素对大鼠C6胶质瘤细胞活性氧线粒体去极化和STAT3、Bcl-2、P53P21表达的影响  

Effects of Grape Seed Proanthocyanidin on ROS,Mitochondrial Depolarization and Expressions of STAT3,Bcl-2,P53,P21mRNA in Rat C6 Glioma Cells

作　　者：陈恒三 路安庆[1] 梁金[1] 金明 魏祎 于向阳 尚银武 黄铭 陈天立[1] CHEN Hengsan;LU Anqing;LIANG Jin;JIN Ming;WEI Yi;YU Xiangyang;SHANG Yinwu;HUANG Ming;CHEN Tianli(Neurosurgery of People’s Hospital of Gansu Province,Lanzhou,Gansu 730000)

机构地区：[1]甘肃省人民医院神经外科,甘肃兰州730000

出　　处：《中国中医药科技》2022年第1期32-35,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：甘肃省科学基金项目(2006)。

摘　　要：目的:探讨葡萄籽原花青素(GSP)对大鼠C6胶质瘤细胞活性氧、线粒体去极化和STAT3、P53、P21、Bcl-2 mRNA表达的影响。方法:采用水溶性四唑盐-1(WST-1)法测定GSP干预后C6胶质瘤细胞系的细胞活力,采用ROS指示剂荧光二氯荧光素二乙酸酯(H2DCFDA)测定干预后C6胶质瘤细胞内ROS水平,采用线粒体膜电位指示剂5,50,6,60-四氯-1,10,3,30-四甲基苯并咪唑碳氰化物碘化物(JC-1)测定干预后C6细胞的线粒体去极化,采用定量PCR法检测GSP干预后C6细胞的STAT3、P53、P21、Bcl-2 mRNA表达。结果:GSP各浓度处理C6胶质瘤细胞去极化细胞百分比、STAT3、P53、P21mRNAbiaoda水平均高于空白组(0 mg/L)(P <0.05),WST-1检测OD值、DCF-MFI和Bcl-2 mRNA水平均低于空白组(0 mg/L)(P <0.05);50 mg/LGSP处理C6胶质瘤细胞去极化细胞百分比、STAT3、P53、P21 mRNA水平均高于25 mg/L(P <0.05),WST-1检测OD值、DCF-MFI和Bcl-2 mRNA水平均低于25 mg/L;75 mg/LGSP处理C6胶质瘤细胞去极化细胞百分比、STAT3、P53、P21 mRNA水平均高于25、50 mg/L(P <0.05),OD值、DCF-MFI和Bcl-2 mRNA水平均低于25、50 mg/L。结论:GSP可以降低C6胶质瘤细胞内ROS水平,诱导线粒体去极化,抑制Bcl-2表达,诱导P21、STAT3和P53表达,抑制胶质瘤细胞增殖效呈剂量依赖趋势。Objective:To approach the effects of Grape Seed Proanthocyanidin( GSP) on rat C 6 glioma cells by observing the changes of ROS,mitochondrial depolarization and expressions of STAT3,Bcl-2,P53,P21 mRNA in C 6 glioma cells.Methods:The proliferation activity of C6 glioma cells was determined by WST-1,ROS was determined by H2 DCFDA,mitochondrial depolarization was determined by JC-1,and RT-PCR was used to examine the expressions of STAT3,Bcl-2,P53,P21 mRNA.Results:The percentage of mitochondrial de-polarization of C6 glioma cells,expressions of STAT3,P53,P21 mRNA in each concentration GSP group were higher than those of control group( P < 0.05),OD value,DCF-MFI and Bcl-2 mRNA expression of C6 glioma cells in each concentration GSP group were lower than those of control group( P < 0.05).Changes of all above indexes at concentration of 50 mg/L were more significant than those at concentration of 25 mg/L,above indexes at concentration of 75 mg/L were more significant than those at concentration of 25,50 mg/L.Conclusion:GSP can decrease ROS in C6 glioma cells,induce mitochondrial depolarization,inhibit Bcl-2 mRNA expression,increase STAT3,P53,P21 mRNA expressions,the inhibition on proliferation of C6 glioma cells was in a dose-dependent manner.

关 键 词：大鼠C6胶质瘤细胞 葡萄籽原花青素 活性氧 线粒体去极化 体外实验 

分 类 号：R285.5[医药卫生—中药学]