基于SLAF_seq的重组自交系群体定位水稻株高QTL  

Mapping of QTLs for Plant Height Using SLAF_seq Recombinant Inbred Lines Population in Rice

作　　者：姜雪 龙武华 彭强 张习春 刘雪薇 张大双 张玉珊[2] 朱速松[1] Jiang Xue;Long Wuhua;Peng Qiang;Zhang Xichun;Liu Xuewei;Zhang Dashuang;Zhang Yushan;Zhu Susong(Guizhou Rice Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang,550006;Guizhou Institute of Bio-technology,Guizhou Academy of Agricultural Sciences,Guiyang,550006)

机构地区：[1]贵州省农业科学院水稻研究所,贵阳550006 [2]贵州省农业科学院生物技术研究所,贵阳550006

出　　处：《分子植物育种》2021年第24期8179-8183,共5页Molecular Plant Breeding

基　　金：贵州省后补助计划项目(黔科合平台人才[2018]5263号);贵州省科技计划项目(黔科合支撑[2019]2304号);贵州省农业科学院青年科学基金项目(黔农科院青年基金[2018]15号);贵州省优质抗病水稻分子育种科技创新人才团队(黔科合平台人才[2018]5620);贵州山区特色优质稻分子育种及品质分析平台建设项目(黔科中引地[2019]4001号)共同资助。

摘　　要：株高是水稻最重要的农艺性状之一,定位和克隆水稻株高基因具有重要的现实意义。本研究利用矮秆品种‘V20B’和高秆品种‘CPSLO17’作为亲本,构建了150个重组自交系家系(RILs),结合SLAF_eq技术构建的水稻高密度遗传图谱,对水稻株高进行QTL检测。利用MapQTL5进行区间作图,阈值设定为2.5,在第1和第6染色体各检测到2个株高QTL位点,分别命名为qPH1-1、qPH1-2、qPH6-1和qPH6-2,这4个QTL位点的LOD值分别为3.2、10.4、3.1和5.6,对表型变异的贡献率分别为6.5%、22.2%、6.0%和11.6%。其中,qPH1-1、qPH1-2和qPH6-2增效位点来自于‘CPSLO17’,而qPH6-1增效位点来自于‘V20B’。本研究为进一步克隆水稻株高QTLs,进而阐明株高的分子调控机制提供参考依据。Plant height is one of the most important agronomic traits of rice,and mapping and cloning the genes controlling the plant height of rice have vital significance.This study used 150 recombinant inbred line(RILs),derived from a cross between’V20 B’and’CPSLO17’.Using a rice high-density genetic linkage map of SLAF makers and interval mapping,performed by MapQTL5,to detect rice plant height QTLs.Two QTLs for plant height were detected on chromosome 1 and 6 respectively,named qPH1-1,qPH1-2,qPH6-1 and qPH6-2,individual QTL(LOD=3.2,LOD=10.4,LOD=3.1 and LOD=5.6)explained 6.5%,22.2%,6.0%and 11.6%of the observed phenotype variance,and the effect-increasing of qPH1-1,qPH1-2 and qPH6-2 loci were originated from’CPSLO17’,while the effect-increasing of qPH6-1 loci was originated from’V20 B’.Therefore,this study provides a reference for further cloning of plant height QTLs,and then to clarify the molecular regulation mechanism of plant height in rice.

关 键 词：水稻(Oryza sativa L.) 重组自交系 株高 

分 类 号：S511[农业科学—作物学]