采用r DNA-ITS和SRAP综合分析黑木耳栽培种的遗传多样性  

Genetic Diversity of Cultivated Auricularia auricula by Comprehensive Analysis of r DNA-ITS and SRAP

作　　者：刘昆昂[1] 马宏[1] 刘萌 张根伟[1] 尹淑丽[1] 李书生[1] Liu Kun'ang;Ma Hong;Liu Meng;Zhang Genwei;Yin Shuli;Li Shusheng(Institute of Biology,Hebei Academy of Sciences,Shijiazhuang,050081)

机构地区：[1]河北省科学院生物研究所,石家庄050081

出　　处：《分子植物育种》2021年第24期8223-8232,共10页Molecular Plant Breeding

基　　金：河北省科学院科技计划项目(19309);河北省科学院高层次人才培养工程项目(2020G05)。

摘　　要：以北方地区主栽的15个黑木耳品种为试材,通过菌株亲和性试验、rDNA-ITS及SRAP聚类分析对15个黑木耳栽培种的遗传多样性进行分析,明确了菌株间的亲缘关系,为黑木耳的栽培、遗传育种和种质资源保护提供理论依据。结果表明,15个黑木耳菌株的拮抗反应大多表现为隆起型和沟壑型等2种不亲和的表现,其中5对黑木耳品种之间没有拮抗反应,而‘冀诱1号’与其他菌株有明显的拮抗反应;r DNA-ITS聚类分析将15个黑木耳分成3个大类,其中‘黑木耳-1’和‘黑木耳-2’、‘黑威伴金’和‘龙江黑+’、‘吊袋’和‘黑山’遗传距离为0,而‘冀诱1号’与其他品种遗传距离较大;筛选出的9对SRAP引物扩增的条带多态性丰富,多态性片段占总数的79.8%,通过聚类分析将15个黑木耳栽培种共分为3大类,其中‘吊袋’和‘黑山’菌株间相似系数为95%,表明它们之间遗传差异很小,‘冀诱1号’与其他菌株间亲缘关系较远。The genetic diversity of 15 Auricularia auricula cultivars in North China was analyzed by strain affinity test,r DNA-ITS and SRAP cluster analysis.The genetic relationship among the strains was clarified,which provided theoretical basis for the cultivation,genetic breeding and germplasm resources protection of A.auricula.The results showed that most of the antagonistic reactions of 15 A.auriculastrains showed two kinds of incompatibility,i.e.non uplift type and ravine type,among which 5 had no antagonistic reaction between A.auricula varieties,while’Jiyou No.1’had obvious antagonistic reaction with other strains.rDNA-ITS cluster analysis divided 15 A.auricula strains into three categories,the genetic distance between including’Heimuer-1’and’Heimuer-2’,’Heiwei banjin’and’Longjianghei+’,’Diaodai’and’Heishan’were 0,while that between’Jiyou No.1’and other varieties was larger.The9 SRAP primers amplified bands were rich in polymorphism,and the polymorphic fragments accounted for 79.8%of the total.The 15 cultivated species of A.auricula were divided into 3 categories by cluster analysis.The similarity coefficient among the strains of’Heiweibanjin’and’Longjianghei+’was 95%,which indicated that there was no genetic difference between them.The genetic relationship between’Jiyou No.1’and other strains was far.

关 键 词：黑木耳(Auricularia auricula) RDNA-ITS SRAP 遗传多样性 

分 类 号：S646.6[农业科学—蔬菜学]