半夏居群遗传多样性及遗传结构分析  

Genetic Diversity and Genetic Structure Analysis of Pinellia ternata(Thunb.)Breit Populations

作　　者：潘凤 莫忠妹 石甜 吴敏 关萍 赵财 Pan Feng;Mo Zhongmei;Shi Tian;Wu Min;Guan Ping;Zhao Cai(Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Life Sciences,Institute of Agro-bioengineering,Guizhou University,Guiyang,550025;Key Laboratory of Biodiversity Conservation and Utilization in Fanjing Mountain Area of Guizhou Province,Tongren University,Tongren,554300)

机构地区：[1]贵州大学生命科学学院,农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵阳550025 [2]铜仁学院,贵州省梵净山地区生物多样性保护与利用重点实验室,铜仁554300

出　　处：《分子植物育种》2021年第24期8347-8352,共6页Molecular Plant Breeding

基　　金：贵州省教育厅教育部重点实验室开放课题(黔教合KY字[2019]033);贵州科技支撑计划项目(黔科合支撑[2019]2451-2号);贵州省生物学国内一流建设学科开放基金(GNYL[2017]009);贵州省梵净山地区生物多样性保护与利用重点实验室开放课题基金资助(黔科合平台人才[2020]2003);国家重点研发计划课题(2016YFC0502604);贵州省中药材现代产业技术体系建设(GZCYTX-03)共同资助。

摘　　要：为了研究中国野生半夏的遗传多样性及谱系结构,揭示其地理分布格局的形成原因,推测该物种潜在的冰期避难所。对分布于中国16个省份20个居群(205个个体)的半夏核基因ITS序列进行PCR扩增、测序分析。结果发现:在所有的半夏居群中共检测到14个单倍型,其中单倍型H3、H6在居群中分布较广。分子方差分析(AMOVA)结果显示,半夏居群的遗传变异主要发生在居群间(75.83%),居群间遗传分化系数(GST=0.7100.05),具有明显的谱系地理结构且基因流较低(Nm=0.019);中性检验结果Tajima’s D为0.37629(P>0.10),Fu’s Fs值为-1.883,结合失配分析的单峰曲线分布表明半夏居群近期经历过小范围快速扩张。研究推测,半夏在第四纪冰期时可能存在多个避难所。In order to study the genetic diversity and pedigree structure of wild Pinellia ternata in China,reveal the causes of its geographical distribution pattern,and speculate its potential ice age refuge.The its sequences of nuclear gene of Pinellia ternata from 20 populations(205 individuals)in 16 provinces of China were amplified by PCR and sequenced.The results showed that:a total of 14 haplotypes were detected in all populations of Pinellia ternata,among which haplotypes H3 and H6 were widely distributed;The results of AMOVA showed that the genetic variation of Pinellia ternate populations mainly occurred among populations(75.83%),and the genetic differentiation coefficient among populations was GST=0.710>NST=0.784(P>0.05),with obvious pedigree structure and low gene flow(Nm=0.019);The results of neutral test showed that Tajima’s D was 0.37629(P>0.10),and Fu’s Fs was-1.883;It is speculated that Pinellia ternata may have multiple refuges during the Quaternary glacial period.

关 键 词：半夏(Pinellia ternata) 谱系地理 遗传多样性 NRDNA 

分 类 号：S567.239[农业科学—中草药栽培]