艾灸胃经穴调控EGFR信号通路对胃癌细胞生长的抑制作用及其机制研究  

Research on the Inhibiting Effects and Its Mechanism of Weijing Acupoint Moxibustion for Regulating EGFR Signal Channel on Gastric Cancer Cells

作　　者：聂瑞华 龚安 NIE Ruihua;GONG An(Qihuang College of Traditional Chinese Medicine,Jiangxi University of Traditional Chinese Medicine,Jiangxi Province,Nanchang 330025,China)

机构地区：[1]江西中医药大学岐黄国医书院,江西南昌330025

出　　处：《中国中医药现代远程教育》2022年第2期136-139,共4页Chinese Medicine Modern Distance Education of China

基　　金：江西省教育厅科学技术厅研究项目【No.GJJ160847】;江西省卫计委中医药科研课题项目【No.51517002】。

摘　　要：目的探讨艾灸胃经穴调控EGFR信号通路对胃癌细胞生长的抑制作用及其机制。方法选取大鼠100只,在检疫3周后采用随机数字表法分为正常组、艾灸胃经穴组、艾灸对照点组、艾灸胃经穴+PD153053组和艾灸对照点+PD153053组,各20只,实验20周后处死制备血清检测相关指标,以EGFR信号通路为切入点,探讨艾灸胃经穴抑制胃癌细胞生长的信号转导机制。结果5组在EGFR基因表达、pERK的Western blot检测结果和c-myc基因表达水平方面对比差异均有统计学意义(P<0.05)。结论EGFR信号通路可以介导癌细胞的增长,而艾灸胃经穴进行干预有助于抑制癌细胞的增殖和迁移,控制病情的发展,干预效果显著。Objective To study the inhibiting effects and its mechanism of gastric cancer cells using Weijing acupoint moxibustion for regulating EGFR(epidermal growth factor receptor)signal channel.Methods After 3 weeks of quarantine,100 rats were randomly divided into normal group,Weijing acupoint moxibustion group,moxibustion group.Weijing acupoint moxibustion+PD153053 group and moxibustion+PD153053 group,with 20 cases in each group.After 20 weeks of trials,serum indicators were measured.By referring to EGFR signal channels,the signal transduction mechanism of Weijing acupoint moxibustion on the inhibition of gastric cancer cells was discussed.Results EGFR gene expressions,pERK(protein kinase R-like endoplasmic reticulum kinase)by Western blot and c-myc gene expressions among five groups were significantly different(P<0.05).Conclusion EGFR signal channel can be mediated into the cancer cell growth.However,Weijing acupoint moxibustion can inhibit the cancer cell proliferation and migration so as to control the disease progression.

关 键 词：胃癌 艾灸疗法 胃经穴 EGFR信号通路 实验研究 

分 类 号：R245[医药卫生—针灸推拿学]