粪菌移植疗法对大鼠脊髓损伤后的作用研究  

Study on the effect of fecal microbiota transplantation therapy on rats after spinal cord injury

作　　者：刘明富 刘槃[1] 宁宇[1] 张琼 农海斌 宗少晖[1] Liu Mingfu;Liu Pan;Ning Yu;Zhang Qiong;Nong Haibin;Zhong Shaohui(The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;School of Public Health,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院,南宁530021 [2]广西医科大学公共卫生学院,南宁530021

出　　处：《广西医科大学学报》2021年第12期2240-2245,共6页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.82060399);广西壮族自治区卫生厅自筹经费科研课题(No.Z2012127)。

摘　　要：目的:探讨粪菌移植疗法(FMT)对大鼠脊髓损伤(SCI)后的作用。方法:将36只SD大鼠随机分为对照组(A组)、SCI组(B组)、FMT组(C组),每组12只。A组仅手术暴露,不进行击打,B组和C组用改良Allen's击打法建立SCI模型。C组灌胃给予粪菌液,连续14 d。对16S rDNA V3-V4高度保守区进行HiSeq平台测序,比较SCI后肠道菌群变化,Basso-Beattie-Bresnahan(BBB)评分评估大鼠后肢情况,酶联免疫吸附试验(ELISA)法检测脊髓组织IL-17、IL-4和IL-10水平,苏木精—伊红(HE)染色法观察脊髓组织病理学变化,尼氏染色观察脊髓神经元细胞及尼氏小体。结果:与A组比较,B组有益菌Bacteroidetes和Saccharibacteria数量减少,BBB评分下降,脊髓组织中抗炎因子IL-10和IL-4下降和促炎因子IL-17升高(均P<0.05),炎性细胞浸润和神经元细胞核固缩,尼氏小体数量明显减少;与B组比较,C组BBB评分升高,脊髓组织IL-10和IL-4含量升高,IL-17含量下降(均P<0.05),细胞变性水肿情况改善,尼氏小体数量增加。结论:FMT疗法能够有效抑制SCI后炎症反应,从而治疗SCI。Objective:To explore the effect of fecal microbiota transplantation(FMT)therapy on rats after spinal cord injury(SCI).Methods:A total of 36 SD rats were randomly divided into control group(group A),SCI(group B),and fecal transplantation group(group C),with 12 rats in each group.Rats in groups B and C were subjected to SCI by modified Allen's percussion method,and group A underwent sham surgery.Group C rats were given fecal bacteria solution by gavage for 14 days.Sequencing of 16S rDNA V3-V4 highly conserved region on HiSeq platform to compare intestinal flora changes after SCI.Basso-Beattie-Bresnahan(BBB)score was used to assess the condition of the hind limbs of rats.Enzyme-linked immunosorbent assay(ELISA)was used to measure spinal cord tissues IL-17,IL-4 and IL-10 levels.The pathological changes of spinal cord tissues were observed by hematoxylin-eosin(HE)staining.The spinal cord neuronal cells and Nissler vesicles were observed by Nissler staining.Results:Compared with group A,SCI led to decrease in the number of beneficial bacteria Bacteroidetes and Saccharibacteria,BBB score,and anti-inflammatory factors IL-10 and IL-4 levels,but an increase in the proinflammatory factor IL-17 level in spinal cord tissue(all P<0.05).Inflammatory cell invasion,neuronal cell nuclear consolidation and few Nisus vesicles were found in group B.Compared with group B,the BBB score and the IL-10 and IL-4 levels in spinal cord tissues were increased,while the IL-17 level was decreased in group C(all P<0.05).Moreover,HE and Nissler staining revealed improved cellular degeneration and edema,and increased Nisus vesicles.Conclusion:Fecal bacteria transplantation therapy can effectively suppress the inflammatory response after SCI and thus treating SCI.

关 键 词：粪菌移植 脊髓损伤 炎症反应 炎性因子 

分 类 号：R651.2[医药卫生—外科学]