低温驯化对菜豆内源多胺含量及其合成分解基因表达的影响  

Effects of Low Temperature Acclimation on Endogenous Polyamine Content and Expression of Biosynthesis and Decomposition Genes in Phaseolus vulgaris L.

作　　者：刘畅[1] 李佳荫 冯国军[1] 刘大军[1] 闫志山[1] 杨晓旭 LIU Chang;LI Jiayin;FENG Guojun;LIU Dajun;YAN Zhishan;YANG Xiaoxu(College of Modern Agriculture and Ecological Environment,Heilongjiang University,Harbin,Heilongjiang 150030)

机构地区：[1]黑龙江大学现代农业与生态环境学院,黑龙江哈尔滨150030

出　　处：《北方园艺》2021年第23期30-37,共8页Northern Horticulture

基　　金：黑龙江省省属高等学校基本科研业务费基础研究资助项目(2020-KYYWF-1026,2020-KYYWF-1026);黑龙江省自然科学基金资助项目(LH2020C090)。

摘　　要：以7个菜豆品种为试材,采用对冷害指数以及相对电解质渗透率进行测定的方式,筛选出耐低温型菜豆品种和低温敏感型菜豆品种,并对其低温驯化过程中内源多胺含量进行测定,研究了腐胺、亚精胺和精胺在响应低温驯化过程中含量的变化,以及对多胺合成分解酶基因表达量的影响,以期为今后菜豆外施多胺缓解低温胁迫提供参考依据。结果表明:在低温驯化过程中2个品种叶片中腐胺和亚精胺的含量有明显增加,而精胺含量变化规律不明显。在低温驯化过程多胺合成酶基因ADC表达量上升,ODC表达量变化不明显,SAMDC表达量升高,SPDS和SPMS在2个品种中表现不一致;多胺分解酶基因DAO和PAO表达量上升,SMO在2个品种中表现不一致。综上所述,腐胺和亚精胺在植物体内的积累可能与提高菜豆苗期耐低温性有较大关系,并且它们的积累可能与ADC、SAMDS、DAO和PAO的表达水平有关。Seven snap beanvarieties were used as test materials.Cold injury index and relative electrolyte permeability were used to screen low temperature tolerant and low temperature sensitive snap bean varieties, and the content of endogenous polyamine in the process of low temperature acclimation was determined to study the changes of putrescine, spermidine and spermine content in response to low temperature acclimation, as well as the effects on gene expression of polyamine synthesis and decomposing enzymes, in order to provide reference for the application of polyamines to alleviate low temperature stress in snap bean in the future.The results showed that the contents of putrescine and spermidine in the leaves of the two cultivars increased significantly during the low temperature acclimation, but the change of spermidine content was not obvious.In the process of low temperature acclimation, the expression of polyamine synthase gene ADC increased, the expression of ODC did not change significantly, the expression of SAMDC increased, SPDS and SPMS were inconsistent in the two varieties;the expression of polyamine lyase gene DAO and PAO increased, SMO was inconsistent in the two varieties.In conclusion, the accumulation of putrescine and spermidine in plants may be related to the improvement of low temperature tolerance of Snap bean seedlings, and their accumulation may be related to the expression levels of ADC,SAMDC,DAO and PAO.

关 键 词：菜豆 多胺 低温驯化 基因表达 

分 类 号：S643.1[农业科学—蔬菜学]