辣木茎提取物对过氧化氢诱导的小鼠晶状体浑浊度的影响  

Effect of Moringa oleifera Stem Extract on Hydrogen Peroxide-induced Opacity of Cultured Mouse Lens

作　　者：钟瑞生 徐清妍 朱晓燕 郭连春 林媛[1] ZHONG Ruisheng;XU Qingyan;ZHU Xiaoyan;GUO Lianchun;LIN Yuan(Department of Ophthalmology,Xiamen Hospital of Traditional Chinese Medicine,Fujian Province,Xiamen 361009,China)

机构地区：[1]厦门市中医院眼科,福建厦门361009

出　　处：《光明中医》2021年第24期4170-4174,共5页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：福建省卫健委青年科研课题(No.2020QNB073)。

摘　　要：目的辣木具有很强的抗氧化性。此研究探究辣木茎提取物(MOSE)对体外培养的小鼠晶状体中氧化应激引起的白内障的潜在影响。方法体外培养的小鼠晶状体经MOSE(0.5 mg/ml和1 mg/ml)预处理24 h,加入1 mmol/L过氧化氢,将小鼠晶状体再培养24 h,之后转入正常培养基,48 h后,测定晶状体组织中的晶状体浑浊度、活性氧(ROS)生成、还原型谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性。结果 MOSE(1 mg/ml)可减轻晶状体浑浊,减少ROS生成,增加GSH含量,提高SOD和CAT活性。此外,MOSE可上调SOD、CAT的表达。结论此研究表明MOSE可减轻氧化应激诱导的白内障形成,其作用机制主要与晶状体内源性抗氧化系统的功能改善有关。Objective Moringa oleifera,also known as horseradish tree or drumstick tree,has strong antioxidant properties.In the present study,we investigated the potential effect of Moringa oleifera stem extract( MOSE) on cataract formation induced by oxidative stress in cultured mouse lenses.Methods Mouse lenses cultured in vitro were pretreated with MOSE(0.5 and 1 mg/ml) for 24 h.Then,1 mmol/L hydrogen peroxide was added,and mouse lenses were cultured for a further 24 h.The medium was then changed to normal culture medium.After 48 h,lens opacification,reactive oxygen species(ROS) generation,reduced glutathione(GSH) content,and activities of superoxide dismutase(SOD) and catalase(CAT) were measured in lens tissues.Results MOSE(1 mg/ml) alleviated lens opacification,reduced ROS generation,increased GSH content,and elevated SOD and CAT activities in cultured lenses.Moreover,MOSE unregulated the expressions of SOD and CAT.Conclusion This study showed that MOSE alleviates oxidative stress-induced cataract formation,and the mechanism of the effect is mainly related to its improvement of the endogenous antioxidant system in the lens.

关 键 词：辣木茎 晶状体 器官培养 氧化应激 抗氧化剂 

分 类 号：R285.5[医药卫生—中药学]