原子吸收光谱法测定聚古罗糖醛酸丙酯硫酸酯钠中的钠含量  

Determination of sodium content in Propylene glycol polyguluronate sulfate sodium by atomic absorption spectrometry

作　　者：李建杰 胡婷[1,2,3] 王箴言 辛萌 郑梦梦[3] 刘晓坤 于广利 LI Jian-jie;HU Ting;WANG Zhen-yan;XIN Meng;ZHENG Meng-meng;LIU Xiao-kun;YU Guang-Li(Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts of Qingdao Pilot National Laboratory for Marine Science and Technology,Qingdao 266237,China;Marine Biomedical Research Institute of Qingdao Co.,Ltd.,Qingdao 266100,China)

机构地区：[1]中国海洋大学山东省糖科学与糖工程重点实验室,医药学院,山东青岛266003 [2]青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,山东青岛266237 [3]青岛海洋生物医药研究院股份有限公司,山东青岛266100

出　　处：《中国海洋药物》2021年第6期49-54,共6页Chinese Journal of Marine Drugs

基　　金：山东省重大科技创新工程专项(2018SDKJ0404-3);山东省泰山产业领军人才工程蓝色人才专项资助。

摘　　要：目的建立聚古罗糖醛酸丙酯硫酸酯钠(PGGS)中钠元素的含量测定方法。方法采用火焰原子吸收分光光度法,以氯化铯为电离抑制剂,分别在589、330 nm 2个吸收波长下进行了PGGS钠含量测定,并进行方法学的比较和验证。结果在589 nm吸收波长下,Na元素在0.4-2.8μg/mL浓度范围内呈良好的线性关系(r=0.999 1);定量限为0.4μg/mL;平均回收率为99.15%(n=9),相对标准偏差(RSD)为1.34%。在330 nm吸收波长下,Na元素在20-200μg/mL浓度范围内呈良好的线性关系(r=0.998 0);定量限为20μg/mL;平均回收率为99.65%(n=9),RSD为2.07%。结论采用原子吸收光谱法测定PGGS中钠元素含量,可根据供试品中Na元素含量高低选择不同的分析波长。该方法结果准确、操作简单快捷,适用于PGGS等褐藻胶硫酸酯衍生物中钠含量的质量控制。Objective To establish a quantitative determination method for sodium ions in Propylene glycol polyguluronate sulfate sodium(PGGS). Methods The sodium content of PGGS was measured by atomic absorption spectroscopy(AAS), caesium chloride was applied as a deionization agent. The wavelengths of 330 nm and 589 nm were compared, and the method was validated. Results The calibration curve was found to be linear between the range of 0.4-2.8 μg/mL for sodium ion(r = 0.999 1) at 589 nm, and the quantitation limit was 0.4 μg/mL. The average recovery was 99.15%, with RSD of 1.34%(n = 9). The calibration curve was found to be linear between the range of 20-200 μg/mL for sodium ion(r = 0.998 0) at 330 nm, and the quantitation limit was 20 μg/mL. The average recovery was 99.65%, with RSD of 2.07%(n = 9). Conclusion The content of sodium in PGGS was determined by atomic absorption spectrometry. The different analysis wavelengths could be selected according to the content of sodium in the test sample. This method was accurate,simple and rapid, and it was suitable for the determination and quality control of the sodium ion in Alginate sulfate derivatives such as PGGS.

关 键 词：火焰原子吸收法 褐藻胶硫酸酯衍生物 钠 

分 类 号：R917[医药卫生—药物分析学]