小檗碱体外对酵母相马尔尼菲篮状菌的抗菌活性检测  

Evaluation of in vitro antifungal activity of berberine against Talaromyces marneffei in yeast phase

作　　者：罗宏 潘开素 张莲 谈园 黄敖 曹存巍[2] Luo Hong;Pan Kaisu;Zhang Lian;Tan Yuan;Huang Ao;Cao Cunwei(Department of Dermatology,The First Hospital of Changsha,Changsha 410005,China;Department of Dermatology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]长沙市第一医院皮肤科,长沙410005 [2]广西医科大学第一附属医院皮肤科,南宁530021

出　　处：《中华皮肤科杂志》2022年第1期55-57,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金(81960567);长沙市卫生计生委2018年科研计划课题项目。

摘　　要：目的体外检测小檗碱对酵母相马尔尼菲篮状菌(TM)的抗菌活性。方法TM标准株(ATCC22019)1株,临床分离株10株,野生竹鼠分离株10株。TM以(1~5)×10^(3)菌落形成单位/ml菌悬液接种于用微量稀释法制备的小檗碱、氟康唑、伊曲康唑、伏立康唑、两性霉素B、卡泊芬净药敏板,在37℃下培养48 h后观察结果,同时设阳性对照(仅加TM和培养基,不加药物)和阴性对照(仅加培养基,不加TM和药物)。参照M27-A3方案测定最低抑菌浓度(MIC)。结果质控株(ATCC22019)MIC均在参考范围内,阳性对照孔TM生长良好。小檗碱MIC范围为32~64 mg/L;氟康唑对非耐药株MIC为2~4 mg/L,对2株临床耐药株为128 mg/L;伊曲康唑MIC为0.06~0.125 mg/L,伏立康唑为0.06~0.125 mg/L,两性霉素B为1~2 mg/L,卡泊芬净为16~32 mg/L。结论小檗碱具有抑制酵母相TM活性的作用。Objective To evaluate the in vitro antifungal activity of berberine against Talaromyces marneffei(TM)in yeast phase.Methods There were 21 TM strains,including l standard strain(ATCC22019),10 clinical isolates and 10 isolates from wild bamboo rats.TM strain suspensions at a concentration of(1-5)×10^(3) colony-forming units/ml were incubated in microdilution plates containing difierent concentrations of berberine,fluconazole,itraconazole,voriconazole,amphotericin B or caspofungin at 37℃for 48 hours.Meanwhile,the wells containing only culture media and TM strains but without antifungal drugs served as the positive control group,and those containing only culture media served as the negative control group.The minimum inhibitory concentrations(MICs)of antifungal drugs against TM yeasts were determined according to the Clinical and Laboratory Standards Institute(CLSI)broth microdilution susceptibility method(M27-A3 document).Results The MICs of the above antifungal drugs were all within the reference ranges for the quality control strain(ATCC22019),and TM strains grew well in the positive control wells.The MIC ranges of berberine,itraconazole,voriconazole,amphotericin B and caspofungin against TM strains were 32-64 mg/L,0.06-0.125 mg/L,0.06-0.125 mg/L,1-2 mg/L and 16-32 mg/L respectively;the MIC range of fluconazole was 2-4 mg/L for non-resistant strains,and 128 mg/L for fluconazole-resistant clinical strains.Conclusion Berberine exhibits antifungal activity against TM in yeast phase.

关 键 词：小檗碱 真菌 微生物敏感性试验 马尔尼菲篮状菌 

分 类 号：R285[医药卫生—中药学]