基于UHPLC-Q-Orbitrap HRMS技术的6-姜酚在大鼠体内代谢产物的鉴定及代谢途径的分析  

Metabolites and metabolic pathway analysis of 6-gingerol in rats based on UHPLC-Q-Orbitrap HRMS

作　　者：孙志[1,2,3] 周霖 禹明洋[1] 王振辉 乔高星 薛鹏 赵红宇 康建[1,2,3] 张晓坚[1,2,3] 左莉华[1,2,3] SUN Zhi;ZHOU Lin;YU Ming-yang;WANG Zhen-hui;QIAO Gao-xing;XUE Peng;ZHAOHong-yu;KANG Jian;ZHANG Xiao-jian;ZUO Li-hua(Department of Pharmacy,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Henan Engineering Research Center of Clinical Mass Spectrometry for Precision Medicine,Zhengzhou 450052,China;Zhengzhou Key Laboratory of Clinical Mass Spectrometry,Zhengzhou 450052,China;Oral Emergency Department,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;College of Medicine,Henan Polytechnic University,Jiaozuo 454000,China)

机构地区：[1]郑州大学第一附属医院药学部,河南郑州450052 [2]河南省精准医学临床质谱工程研究中心,河南郑州450052 [3]郑州市临床质谱重点实验室,河南郑州450052 [4]郑州大学第一附属医院口腔急诊科,河南郑州450052 [5]河南理工大学医学院,河南焦作454000

出　　处：《中草药》2021年第24期7420-7427,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81703666);河南省高等学校重点科研项目基础研究计划(18A310036,19A360008,19A320070)。

摘　　要：目的采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UHPLC-Quadrupole-Orbitraphighresolutionmass spectrometry,UHPLC-Q-Orbitrap HRMS)技术对6-姜酚在大鼠血浆、尿液和粪便中的代谢产物进行鉴定分析。方法 SD大鼠ig给药6-姜酚羧甲基纤维素钠混悬液后,收集其血浆、尿液、粪便以及胆汁样品。样品前处理后,采用Waters Acquity UPLC?BEH C18色谱柱(100 mm×2.1 mm,1.7μm);以0.1%甲酸水溶液(A)-乙腈(B)为流动相,梯度洗脱。在电喷雾电离源(ESI源)正、负离子模式下采集生物样品的质谱数据。结果对代谢产物的质谱数据进行结构鉴定分析,检测到大鼠体内6-姜酚相关代谢产物共21个,其中血浆样品中5个代谢产物,尿液样品中9个代谢物,粪便样品中12个代谢产物,胆汁样品中8个代谢产物。结论通过液质联用技术阐明了6-姜酚在大鼠体内的代谢产物及其代谢途径,为今后相关研究提供重要的理论依据,同时也为药物代谢鉴定研究提供一种综合研究方法。Objective To identify the metabolites of 6-gingerol in plasma, urine, feces and bile in rats by using UHPLC-Q-Orbitrap HRMS method. Methods The plasma, urine, fecal and bile samples were collected and processed after ig administration of 6-gingerol CMC-Na suspension. A Waters ACQUITY UPLC BEH C18 column(100 mm × 2.1 mm, 1.7 μm) was applied and 0.1% formic acid(A)-acetonitrile(B) solution were as the mobile phase for gradient elution. The mass spectrometry data of biological samples was acquired under the both positive and negative electrospray ion mode. Results A total of 21 metabolites of 6-gingerol were identified in rats by analyzing the mass spectrometry data, including five metabolites in plasma, nine metabolites in urine, 12 metabolites products in feces, eight metabolites in bile samples. Conclusion The study expounded metabolites and metabolic pathways of 6-gingerol in rats based on UHPLC-Q-Orbitrap HRMS method and provided an important basic for future studies. In addition, this paper could offer a comprehensive research method for drug metabolism identification.

关 键 词：6-姜酚 UHPLC-Q-Orbitrap HRMS 代谢产物 代谢途径 血浆 尿液 粪便 胆汁 

分 类 号：R284.1[医药卫生—中药学]