HPLC-CAD法测定不同产地和花期闹羊花中闹羊花毒素Ⅱ、Ⅲ、Ⅴ  

Simultaneous determination of rhodojaponins Ⅱ, Ⅲ, Ⅴ of Rhododendri Mollis Flos from different producing area and flowering phase by HPLC-CAD

作　　者：冯靖雯 郭小红 李丹[1] 胡媛[1] 刘友平[1] 陈鸿平[1] FENG Jing-wen;GUO Xiao-hong;LI Dan;HU Yuan;LIU You-ping;CHEN Hong-ping(College of Pharmacy,Chengdu University of Traditional Chinese Medicine Key Laboratory of Standardization of Traditional Chinese Medicine Ministry of Education State Key Laboratory of Traditional Chinese Medicine Resources with Southwest Characteristics,Chengdu,611137,China)

机构地区：[1]成都中医药大学药学院中药标准化教育部重点实验室西南特色中药资源国家重点实验室(筹),四川成都611137

出　　处：《中草药》2021年第24期7624-7629,共6页Chinese Traditional and Herbal Drugs

基　　金：国重·双一流·中药质量保障提升创新科研团队(030041011);重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0552);重庆市科研院所绩效激励引导专项(cstc2018jxjl130011);成都中医药大学杏林学者学科人才科研提升计划(YYZX20180023)。

摘　　要：目的建立闹羊花中闹羊花毒素Ⅱ、Ⅲ、Ⅴ的定量分析方法,并测定不同产地、花期闹羊花毒素含量,用于闹羊花药材的质量评价及产地、采收期的选择。方法采用Waters C18色谱柱(250 mm×4.6 mm,5μm)色谱柱,甲醇(A)-水(B)流动相,建立高效液相色谱联合电雾式检测器(HPLC-CAD)方法,并进行方法学验证,对17批不同产地、5批不同花期药材中3种闹羊花毒素进行含量测定,并聚类分析。结果所建立的分析方法简便,准确度和精密度良好,能作为闹羊花药材常规定量评价方法;不同产地闹羊花药材可聚为3类,第1类(湖南永州、郴州、岳阳,湖北黄石及山东日照)闹羊花毒素Ⅴ含量明显低于其他地区,第2类(江西上饶、抚州与2批购自湖北荷花池中药材市场,1批湖北随州)3种闹羊花毒素总含量低于其他2类,第3类(湖北随州和安徽岳西、霍山)3种成分含量均较高。不同花期闹羊花中闹羊花毒素Ⅱ、Ⅲ盛开末期达最高,闹羊花毒素Ⅴ以半开期最高,3种闹羊花毒素总含量在花蕾期最低,盛开末期最高。结论闹羊花毒素Ⅱ、Ⅲ、Ⅴ含量与产地、花期有相关性,可为药材质量标准的完善提供依据和为产地、采收期的选择提供参考依据。Objective This study was established to simultaneously determine the content of rhodojaponins Ⅱ, Ⅲ, and Ⅴ of Rhododendri Mollis Flos, and to compare samples from different origins and flowering stages. Methods Using Waters C18 column(250 mm × 4.6 mm, 5 μm) column, methanol(A)-water(B) to establish a high performance liquid chromatography combined with electrospray detector(HPLC-CAD) method, and methodological verification was carried out to determine the content of three kinds of rhodojaponin in 17 batches of different origin and five batches of medicinal materials at different flowering dates, and cluster analysis. Results The established analytical method was simple, with good accuracy and precision, and can be used as a routine quantitative evaluation method for Rhododendri Mollis Flos medicinal materials;Samples from different origins can be grouped into three categories, category 1(Yongzhou, Chenzhou, Yueyang in Hunan, Huangshi in Hubei and Rizhao in Shandong) rhodojaponin V was significantly lower than that in other regions. The total content of rhodojaponin in category 2(Jiangxi Shangrao, Fuzhou and two batches purchased from Hehuachi, one batch in Suizhou, Hubei) was lower than that of the other two categories. The content of the three components in the three categories(Suizhou, Hubei, Yuexi, Huoshan, Anhui) was relatively high. Rhodojaponin Ⅱ and Ⅲ were the highest in the late blooming period, rhodojaponin V was the highest in the half-open period, and the total content of the three rhodojaponin is the lowest in the flower bud period and the highest in the late blooming period. Conclusion The content of rhodojaponin Ⅱ, Ⅲ, and Ⅴ is related to the origin and flowering period, which can provide a basis for improving the quality standards of medicinal materials and a reference for the choice of origin and harvesting period.

关 键 词：闹羊花 HPLC-CAD 质量评价 闹羊花毒素Ⅱ 闹羊花毒素Ⅲ 闹羊花毒素Ⅴ 

分 类 号：R286.2[医药卫生—中药学]