小花清风藤茎叶化学成分及其保肝活性研究  

Study on chemical constituents from tems and leaves of Sabia parviflora and their hepatoprotective activity

作　　者：李曼姝 柯银铅 张紫琴 刘呈雄 邹坤 LI Man-shu;KE Yin-qian;ZHANG Zi-qin;LIU Cheng-xiong;ZOU Kun(Hubei Key Laboratory of Natural Products Research and Development,College of Biological and Pharmaceutical Sciences,Three Gorges University,Yichang 443002,China)

机构地区：[1]三峡大学生物与制药学院,天然产物研究与利用湖北省重点实验室,湖北宜昌443002

出　　处：《中草药》2021年第23期7096-7104,共9页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金青年科学基金项目(81803383);水利部水土保持植物开发管理中心合作项目(SDHZ2019030)。

摘　　要：目的研究小花清风藤Sabiaparviflora茎叶的化学成分及其保肝护肝活性。方法采用正相、反相硅胶柱色谱、凝胶柱色谱和半制备液相色谱等方法分离纯化,通过NMR、MS等技术鉴定化合物的结构。结果从小花清风藤茎叶中分离得到22个化合物,分别鉴定为N-反式-阿魏酰酪胺(1)、油酸(2)、(Z)-1,8-heptadeca diene(3)、N-反式-对香豆酰基去甲辛弗(4)、(Z)-N-formyl-nornuciferin(5)、N-demethyl-N-formyldehydronuciferine(6)、N-trans-cinnamoyltyramine(7)、油酸酰胺(8)、1,2,4-trimethoxybenzene(9)、2-(3-methoxyphenyl) isoindoline-1,3-dione(10)、景洪哥纳香胺酮(11)、squamolone(12)、桦木酸甲酯(13)、白桦脂酸(14)、3-氧代齐墩果酸(15)、牛蒡子苷(16)、2-(3′′,4′′-dimethoxybenzyl)-3-(3′-methoxy-4′-hydroxybenzyl)-γ-butyrolactone(17)、matairesinol(18)、lappaol F(19)、山柰酚(20)、lappaol A(21)和lappaol B(22)。结论化合物2、3、5~8、10~12和16~19为首次从清风藤属植物中分离得到。其中化合物8、11、13、14、16、20和22有显著的保肝护肝活性。Objective To study the chemical composition from the stems and leaves of Sabia parviflora and their liver-protecting activity. Methods Normal phase, reverse phase silica gel column chromatography, gel column chromatography and semi-preparative liquid chromatography were used for separation and purification, and the structures of the isolated compounds were identified by NMR and MS. Results Twenty-two compounds were isolated from the stems and leaves of S. parviflora, including N-trans-feruloyl tyramine(1), oleic acid(2),(Z)-1, 8-Heptadeca diene(3), N-trans-p-coumaroyl octopamine(4),(Z)-N-formylnornuciferin(Z)(5), N-demethyl-N-formyldehydronuciferine(6), N-trans-cinnamoyltyramine(7), oleamide(8), 1,2,4-trimethoxybenzene(9), 2-(3-methoxyphenyl)isoindoline-1,3-dione(10), cheliensisaminone(11), squamolone(12), betulinic acid methyl ester(13), betulinic acid(14), 3-oxooleanolic acid(15), arctiin(16), 2-(3′′,4′′-dimethoxybenzyl)-3-(3′-methoxy-4′-hydroxybenzyl)-γ-butyrolactone(17), matairesinol(18), lappaol F(19), kaempferol(20), lappaol A(21), and lappaol B(22).Conclusion Compounds 2, 3, 5—8, 10—12, and 16—19 are isolated from Sabia genus for the first time. Among them, compounds 8, 11, 13, 14, 16, 20, and 22 have showed significant hepatoprotective activities.

关 键 词：小花清风藤 保肝护肝 油酸酰胺 景洪哥纳香胺酮 白桦脂酸 牛蒡子苷 

分 类 号：R284.1[医药卫生—中药学]