基于UPLC-TQ-MS的地黄中核苷类和氨基酸类成分动态积累研究  

Analysis of dynamic accumulation of nucleosides and amino acids in Rehmannia glutinosa based on UPLC-TQ-MS

作　　者：徐卓 戴新新 宿树兰[1] 严辉[1] 郭盛[1] 魏丹丹[1] 钱大玮[1] 段金廒[1] XU Zhuo;DAI Xin-xin;SU Shu-lan;YAN Hui;GUO Sheng;WEI Dan-dan;QIAN Da-wei;DUAN Jin-ao(Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,State Administration of Chinese Medicinal Resource Recycling Utilization,Jiangsu Key Laboratory of High-tech Research of Prescriptions,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心中药资源产业化与方剂创新药物国家地方联合工程研究中心国家中医药管理局中药资源循环利用重点研究室江苏省方剂高技术研究重点实验室,江苏南京210023

出　　处：《中草药》2021年第23期7323-7330,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81673533);国家自然科学基金资助项目(81973708);江苏省科技计划项目(社会发展)(BE2019722)。

摘　　要：目的分析评价地黄Rehmannia glutinosa根与叶中5种核苷与14种氨基酸类成分,揭示其动态积累规律,为其合理利用提供科学依据。方法选取不同生长期的地黄根和叶以及不同产地(河南大封、河南温县、河南南召)的地黄叶为研究对象,采用超高效液相色谱-三重四极质谱联用技术(ultra-high performance liquid chromatography-triple quadrupole mass spectrometry,UPLC-TQ-MS)分析不同生长期地黄根和叶以及不同产地地黄叶中核苷类与氨基酸类成分的动态变化。结果研究发现地黄根及地黄叶中均含有5种核苷类成分和14种氨基酸类成分,2部位中不同生长期2类成分的总含量分别为3.88~16.97 mg/g和4.27~25.32 mg/g;地黄根和叶中测得的2类成分总量分别在10月中下旬和11月上中旬显著升高,达到各自峰值,其中L-谷氨酰胺与L-赖氨酸含量最高,占总含量的60%以上;大部分生长期地黄叶中核苷类和氨基酸类成分较地黄根高,尤其在10月下旬;不同产地地黄叶中以河南南召产者核苷和氨基酸含量较高,总含量最高可达30.756 mg/g。结论阐明了地黄中核苷类和氨基酸类成分的分布和动态积累过程,为地黄药食两用特性及合理采收期提供了科学依据,提出了地黄叶可作为新的食用新资源为机体补充核苷类和氨基酸类成分的建议,为地黄叶资源价值的发现、开发和利用提供了支撑。Objective To investigate the dynamic accumulation of five nucleosides and 14 amino acids in Dihuang(Rehmannia glutinosa)and reveal their dynamic accumulation rules,so as to provide scientific basis for their rational utilization.Methods Leaves and roots from R.glutinosa at different growth stages and from different cultivation regions(Henan Dafeng,Henan Nanzhao,Henan Wenxian)were determined using ultra-high performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-TQ-MS)to analyze the dynamic accumulation of nucleosides and amino acids in leaves and roots of R.glutinosa from different cultivation regions.Results R.glutinosa leaves and roots were all found to contain five kinds of nucleotides and 14 kinds of amino acids,and the total content of those in different growing periods was 3.88—16.97 mg/g and 4.27—25.32 mg/g,respectively.The content of nucleosides and amino acids in R.glutinosa leaves and roots increased significantly in the second half of October and the first and middle of November,reaching their respective peaks.The content of L-glutamine and L-lysine was the highest,accounting for more than 60%.In most growing periods,the content of nucleosides and amino acids in R.glutinosa leaves was higher than that in roots,especially in late October.The content of nucleosides and amino acids were higher in the leaves from Henan Nanzhao,and the total content was up to 30.756 mg/g.Conclusion The distribution and dynamic accumulation process of nucleosides and amino acids in R.glutinos were described.A scientific basis for the medicinal and edible properties and the reasonable harvest period of R.glutinosa was provided.It was suggested that R.glutinosa leaves could be used as a new edible resource to supplement nucleosides and amino acids,which provided support for the discovery,development,and utilization of the resources in R.glutinosa leaves.

关 键 词：地黄 地黄叶 核苷 氨基酸 动态积累 超高效液相色谱-三重四极质谱联用技术 

分 类 号：R286.2[医药卫生—中药学]