当归多糖对造血干细胞SIRT1、p53和p21表达的影响  

Effect of angelica sinensis polysaccharide on expression of SIRT1, p53 and p21 in hematopoietic stem cells

作　　者：李红辉 唐石欢 张先平[2] 龙婷 彭露 张慧 吴沁园 张红梅 王亚平[3] LI Hong-hui;TANG Shi-huan;ZHANG Xian-ping;LONG Ting;PENG Lu;ZHANG Hui;WU Qin-yuan;ZHANG Hong-mei;WANG Ya-ping(Department of Traditional Chinese Medicine,Loudi Central Hospital,Loudi 417000,Hunan,China;不详)

机构地区：[1]娄底市中心医院中医科,湖南娄底417000 [2]娄底市中心医院生殖中心,湖南娄底417000 [3]重庆医科大学干细胞与组织工程研究室,重庆400016

出　　处：《广东医学》2021年第12期1437-1441,共5页Guangdong Medical Journal

基　　金：国家自然科学基金项目(81173398);湖南省科技创新计划项目(2017SK51201);湖南省卫生计生委科研计划课题项目(B20180420)。

摘　　要：目的研究当归多糖(ASP)基于辐射所致小鼠衰老造血干细胞(HSCs) SIRT1、p53和p21蛋白表达的影响,探讨ASP基于调控HSCs衰老的可能机制。方法 72只SPF级C57BL/6J小鼠随机纳入对照组、模型组和ASP组。模型组通过X线3.0G y/8F全身照射建立小鼠HSCs衰老模型;ASP组在照射期间给予ASP灌胃;对照组与模型组予生理盐水。经免疫磁珠法分选小鼠HSCs,采用流式细胞术检测细胞周期,通过β-半乳糖苷酶(SA-β-Gal)染色观察衰老细胞;经混合集落培养(CFU-Mix)观察HSCs自我更新及定向分化潜能;Western blot检测SIRT1、p53、p21蛋白表达。结果与对照组比较,X线照射可能显著增加模型组HSCs G;期细胞比例、SA-β-Gal染色阳性细胞率、p53及p21蛋白表达(P<0.05),降低SIRT1表达和CFU-Mix形成能力(P<0.05)。与模型组比较,ASP会抑制衰老HSCs G;期细胞比例、SA-β-Gal染色阳性细胞率、p53及p21蛋白表达增加(P<0.05),同时SIRT1表达增加、CFU-Mix能力增强(P<0.05)。结论 ASP可能上调SIRT1表达、下调p53及p21表达,从而可能延缓小鼠HSCs衰老。Objective To investigate the effects of angelica sinensis polysaccharide(ASP) based on the expression of HSCs SIRT1, p53 and p21 protein in hematopoietic stem cells(HSCs) of rats with radiation-induced aging, and to discusses possible mechanisms on HSCS aging. Methods Seventy-two SPF grade C57 BL/6 J mice were randomly incorporated into the control group, model group and ASP group.The model group was subjected to a mouse HSCs aging model by X-ray 3.0 GY/8 F system. ASP group was given with ASP during the irradiation. The model and control groups were given with physiological saline. The mouse HSCs were sorted by immunoprugal beads;the cell cycle was detected by flow cytometry;and aging cells were observed by β-galactosidase(Sa-β-Gal) staining. HSCs was observed by mixed colony culture(CFU-Mix)self-update and directional differentiation potential. Western blot was applied for assessing the SIRT1, p53, p21 protein expression. Results Compared to the control group, X-ray irradiation might significantly increase the proportion of the HSCS G;period, SA-β-Gal staining positive cell ratio, p53 and p21 protein expression(P<0.05), reduce SIRT1 expression and CFU-MIX formation ability in model group(P<0.05). Compared with the model group, ASP significantly suppressed the proportion of aging HSCS G;cells, increased the expression of SA-β-Gal staining positive cells, p53 and p21 protein(P<0.05);increased and the expression of SIRT1 and CFU-MIX ability(P<0.05). Conclusion The ASP may up-regulate the expression of SIRT1, and down-regulate p53 and p21 expression, which may delay mice HSCs aging.

关 键 词：当归多糖 SIRT1 造血干细胞 细胞衰老 p53/p21 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] Q255[医药卫生—基础医学]