抑制长非编码RNA配对盒8反义RNA 1对心肌缺血再灌注细胞氧化应激和细胞凋亡水平的影响  

Effect of long non-coding RNA PAX8-AS1 inhibition on oxidative stress and apoptosis of myocardial ischemia-reperfusion cells

作　　者：朱新华[1] 侯静雯 刘蓓蓓[1] 许慧娟 李秋影 张晓阳[1] ZHU Xin-hua;HOU Jing-wen;LIU Bei-bei;XU Hui-juan;LI Qiu-ying;ZHANG Xiao-yang(Department of Geriatrics,The Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学第五附属医院老年病科,乌鲁木齐830000

出　　处：《岭南心血管病杂志》2021年第6期727-730,756,共5页South China Journal of Cardiovascular Diseases

摘　　要：目的探讨抑制长非编码RNA配对盒8反义RNA 1(long non-coding RNA PAX8-AS1,lncRNA PAX8-AS1)对心肌缺血再灌注(myocardial ischemia-reperfusion,MI/R)细胞氧化应激和细胞凋亡水平的影响。方法以H9c2心肌细胞为研究对象,将细胞分为4组:空白对照组、MI/R组、si-con组、si-PAX8-AS1组。实时定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)检测各组心肌细胞PAX8-AS1的表达水平;化学比色法检测各组细胞丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)浓度;流式细胞术检测心肌细胞凋亡率;Western blot检测各组细胞B细胞淋巴瘤-2(B-cell lymphoma-2,Bcl-2)、Bcl-2相关X蛋白质(Bcl-2 associated X protein,Bax)蛋白表达。结果与空白对照组(1.00±0.06)相比,MI/R组(2.63±0.08)、si-con组(2.59±0.04)、si-PAX8-AS1组PAX8-AS1 mRNA表达水平(1.41±0.03)均显著升高,差异有统计学意义(F=687.811,P<0.05);与si-con组相比,si-PAX8-AS1组PAX8-AS1 mRNA表达水平显著降低,差异有统计学意义(LSD-t=27.281,P<0.05)。与空白对照组相比,MI/R组、si-con组、si-PAX8-AS1组MDA浓度、心肌细胞凋亡率及Bax蛋白表达均显著升高,SOD、GSH-Px浓度及Bcl-2蛋白表达均显著降低,差异有统计学意义(均P<0.05);与si-con组相比,si-PAX8-AS1组MDA浓度、心肌细胞凋亡率及Bax蛋白表达降低,SOD和GSH-Px浓度及Bcl-2蛋白表达均升高,差异有统计学意义(均P<0.05)。结论抑制PAX8-AS1表达后可抑制心肌MI/R损伤细胞氧化应激水平并降低细胞凋亡水平。Objectives To investigate the effect of long non-coding RNA PAX8-AS1(lncRNA PAX8-AS1)inhibition on oxidative stress and apoptosis of cells in myocardial ischemia-reperfusion(MI/R).Methods H9c2 cardiomyocytes were divided into four groups:blank control group,MI/R group,si-con group and si-PAX8-AS1 group.The expression of PAX8-AS1 in cardiomyocytes of each group was detected by quantitative real-time polymerase chain reaction(qRTPCR).The content of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in cells of each group was detected by chemical colorimetry.Flow cytometry was used to detect apoptosis rate of cardiac myocytes.The expression of B-cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax)protein in each group was detected by Western blot.Results Compared with blank control group(1.00±0.06),the expression level of PAX8-AS1 mRNA in MI/R group(2.59±0.04),si-con group(2.63±0.08)and si-PAX8-AS1 group(1.41±0.03)were signifi⁃cantly higher(F=687.811,P<0.05);compared with si-con group,the expression level of PAX8-AS1 mRNA in si-PAX8-AS1 group was significantly lower(LSD-t=27.281,P<0.05).Compared with blank control group,MDA con⁃tent,apoptosis rate,Bax protein expression in MI/R group,si-con group and si-PAX8-AS1 group were significantly higher;SOD,GSH-Px content,Bcl-2 protein expression were significantly lower(all P<0.05).Compared with si-con group,the MDA content,apoptosis rate,Bax protein expression decreased;the SOD and GSH-Px content,Bcl-2 pro⁃tein expression increased in si-PAX8-AS1 group(all P<0.05).Conclusions Inhibition of PAX8-AS1 expression can inhibit the oxidative stress level and decrease the apoptosis level of myocardial ischemia-reperfusion injury cells.

关 键 词：长非编码RNA配对盒8反义RNA 1 心肌缺血再灌注 氧化应激 细胞凋亡 

分 类 号：R33[医药卫生—人体生理学]