基于水稻RIL群体的稻瘟病抗性QTL分析  

QTL Mapping of Rice Blast Resistance Based on RIL Population in Rice

作　　者：张小花 田蕾[1] 罗成科 马天利 李培富[1] 孙建昌[2] 张银霞[1] ZHANG Xiaohua;TIAN Lei;LUO Chengke;MA Tianli;LI Peifu;SUN Jianchang;ZHANG Yinxia(College of Agriculture,Ningxia University,Yinchuan 750021,China;Institute of Crop Sciences,Ningxia Academy of Agricultural and Forestry Sciences,Yongning 750105,China)

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏农林科学院农作物研究所,宁夏永宁750105

出　　处：《河南农业科学》2021年第11期97-107,共11页Journal of Henan Agricultural Sciences

基　　金：宁夏回族自治区科技重大专项(2018NYYZ0303)。

摘　　要：为了挖掘和利用抗稻瘟病基因,培育抗稻瘟病品种,以抗稻瘟病亲本京宁11(父本)和感稻瘟病亲本2013ZJP-3(母本)培育的包含189份株系的F6和F7重组自交系(RIL)群体作为试验材料,利用99对亲本间有多态性的SSR标记构建遗传连锁图谱;同时,利用宁夏地区优势稻瘟病菌生理小种,采用人工接种和自然诱发相结合的方法对其进行叶瘟、穗颈瘟抗性鉴定,并用QTL IciMapping 4.0软件对叶瘟抗性和穗颈瘟抗性进行QTL分析。结果表明,F7群体的抗叶瘟性状呈连续性分布,且大部分材料偏向于抗病亲本京宁11。QTL定位结果显示,共检测到11个QTL:与叶瘟抗性有关的QTL有4个,分别位于6、6、6、10号染色体上,贡献率达到5%~8%,其中贡献率最大的QTL位于6号染色体上;与穗颈瘟抗性有关的QTL有4个,分别位于6、10、10、11号染色体上,贡献率为5%~8%;与综合抗病指数有关的QTL有3个,位于6、10、10号染色体;此外,在10号染色体上的RM1125单标记区间内有2个控制穗颈瘟与综合抗病指数的QTL,分别解释了8%、6%的表型变异,加性效应分别为-0.75、-0.40,这个QTL对应的增效基因均来自于父本京宁11。In order to continuously explore and utilize resistance genes and cultivate resistant varieties,in this study,with F6 and F7 recombinant inbred line(RIL) populations containing 189 strains as experimental materials,which were produced by the rice blast resistant parent Jingning 11(male parent)and the rice blast susceptible parent 2013 ZJP-3(female parent),99 pairs of SSR molecular markers with polymorphism among the parents were used to construct a genetic linkage map. At the same time,the resistance of RILs together with their two parents to rice blast was identified by artificial inoculation and natural induction using superior races of rice blast in Ningxia. The QTLs for rice resistance to leaf blast and spike neck blast were detected by IciMapping 4. 0 software. The results showed that leaf blast resistance of F7 populations displayed continuous distribution,and most materials were biased towards the resistant parent Jingning 11. According to QTL localization analysis,a total of 11 QTL sites were detected.There were four QTLs related to leaf blast resistance,located on chromosomes 6,6,6,10,with contribution rate of 5% to 8%. The QTL with the highest contribution rate was located on chromosome 6. There were four QTLs related to spike neck blast resistance,located on chromosomes 6,10,10,11,with contribution rate of5% to 8%. There were three QTLs related to comprehensive resistance index,located on chromosomes 6,10,10. In addition,two loci were found for spike neck blast resistance and comprehensive resistance index in the marker RM1125 on chromosome 10,explaining 8% and 6% of phenotypic variation,and the additive effects were-0. 75,-0. 40. The synergistic gene of the QTL was from the male parent Jingning 11.

关 键 词：水稻 重组自交系群体 稻瘟病 叶瘟 穗颈瘟 QTL分析 

分 类 号：S332.2[农业科学—作物遗传育种]