抑制电导离子色谱法测定蔬菜、药品和水中对氨基苯磺酸  

Determination of 4-aminobenzenesulfonic acid in vegitable,pharmaceuticals and water by suppressed conductivity ion chromatography

作　　者：杜宏山[1] 黄选忠[1] 汪波 张丽[1] 舒开继[1] 邹绍仙 DU Hongshan;HUANG Xuanzhong;WANG Bo;ZHANG Li;SHU Kaiji;ZOU SHaoxian(Xingshan Center for Disease Control and Prevention,Xingshan 443700,China;Department of Laboratory Medicine,Xingshan County People's Hospital,Xingshan 443700,China)

机构地区：[1]湖北省兴山县疾病预防控制中心,湖北兴山443700 [2]湖北省兴山县人民医院,湖北兴山443700

出　　处：《化学分析计量》2022年第1期49-53,共5页Chemical Analysis And Meterage

摘　　要：建立抑制电导离子色谱法测定蔬菜、药品和水中对氨基苯磺酸。以SH-AC-3型阴离子交换柱为分离柱,5.0 mmol/L Na2CO3-1.5 mmol/L NaHCO3为淋洗液,以1.0 mL/min的流量等度洗脱,对氨基苯磺酸与常见阴离子可实现完全分离,对氨基苯磺酸的色谱峰面积与质量浓度在0.05~100.0 mg/L范围内线性关系良好,相关系数为0.9999。该方法应用于蒜苗、复方磺胺甲噁唑片、对氨基苯磺酰胺及水中对氨基苯磺酸的测定,样品加标回收率为95.0%~98.4%,5次平行测定值的相对标准偏差为0.92%~3.23%。按3倍信噪比计算对氨基苯磺酸的检出限为0.04 mg/L。该方法选择性好,操作简便。A new method for the determination of 4-aminobenzenesulfonic acid in vegetables,pharmaceuticals and water by ion chromatography was developed.A SH-AC-3 anion exchange column was used as the separation column,5.0 mmol/L Na2CO3-1.5 mmol/L NaHCO3 was used as the eluting liquid,and 1.0 mL/min flow rate was used to elute the 4-aminobenzenesulfonic acid.There was a good linear relationship between the peak area and the mass concentration of 4-aminobenzenesulfonic acid in the range of 0.05-100.0 mg/L with the corelation coefficient of 0.9999.The method was applied to the determination of 4-aminobenzenesulfonic acid in garlic sprouts,sulfamethoxazole tablets,p-aminobenzenesulfonamide and water.The recoveries were 95.0%-98.4%and the relative standard deviation of 5 parallel determinations was 0.92%-3.23%.The detection limit of 4-aminobenzenesulfonic acid was 0.04 mg/L based on the calculation of signal to noise ratio of 3.The method has high selectivity,and it is simple to operate.

关 键 词：离子色谱法 对氨基苯磺酸 蔬菜 药品 水 

分 类 号：O657.7[理学—分析化学]