opaque-2基因导入甜糯玉米表型分析及导入系群体初步创制  

Phenotype Analysis of opaque-2 Gene Introduced into Sweet-waxy Maize and Preliminary Construction of Near-isogenic Lines

作　　者：张惠惠 李余良[1] 刘建华[1] ZHANG Huihui;LI Yuliang;LIU Jianhua(Crops Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Crops Genetics&Improvement,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室,广东广州510640

出　　处：《广东农业科学》2021年第12期9-15,共7页Guangdong Agricultural Sciences

基　　金：广东省现代农业产业技术体系创新团队项目(2020KJ106,2021KJ106);广东省农业科学院农业优势产业学科团队建设项目(202115TD)。

摘　　要：【目的】甜糯玉米是华南地区重要的经济作物,但籽粒中赖氨酸含量低,探索研究opaque2(o2)导入对甜糯玉米籽粒表型及赖氨酸积累的影响。【方法】通过常规杂交方法在甜糯玉米中引入o2基因,利用o2基因内紧密连锁的SSR共显性标记检测供体和甜糯玉米受体自交系的多态性,利用其特异性和共显性检测回交自交后代。【结果】投射光下供体亲本HP3籽粒表现不透光,受体材料透光性良好;杂交穗当代表现不同基因型籽粒分离,出现硬质、皱缩等表型,回交子代籽粒透光性明显降低,可能与o2基因存在有关。对o2基因的3对SSR标记umc1066、phi112和phi057筛选,只有标记phi057在供体和受体自交系中呈现良好的多态性,进一步研究发现,在回交和自交后代中能够检测到供体的o2突变基因成功导入甜糯玉米受体自交系。【结论】通过子代籽粒表型及SSR标记phi057筛选鉴定,初步创制了Opaque-2甜/糯玉米早代群体系;SSR标记phi057筛选出F1与BC1代o2基因植株,说明phi057标记可有效区分含o2基因植株。【Objective】Sweet-waxy maize is an important cash crop in South China,but the content of lysine in its grain is low.The effects of opaque2(o2)introduction on grain phenotype and lysine accumulation of sweet-waxy maize were studied.【Method】The o2 gene was introduced into sweet-waxy maize by conventional hybridization,the polymorphism of donor and sweet-waxy maize receptor inbred lines was detected by SSR codominant markers closely linked in o2 gene,and the backcross inbred offspring were detected by its specificity and codominant.【Result】Under projection light,the seeds of donor parent HP3 were opaque,and the light transmittance of recipient material was good.The hybrid panicle showed grain separation of different genotypes,hard and wrinkled phenotypes,and the grain light transmittance of backcross offspring decreased significantly,which may be related to the existence of o2 gene.Through the screening of three pairs of SSR markers umc1066,phi112 and phi057 of o2 gene,only marker phi057 showed good polymorphism in donor and recipient inbred lines.Further study found that the o2 mutant gene of donor could be detected in backcross and inbred offspring,and successfully introduced into sweet-waxy maize receptor inbred lines.【Conclusion】The early generation group system of o2 sweet/waxy maize was preliminarily created.The o2 gene plants of F1 and BC1 were screened by SSR marker phi057,indicating that phi057 marker ca n effectively distinguish o2 gene plants.

关 键 词：Opaque-2基因 甜糯玉米 分子标记辅助选择 近等基因系 赖氨酸 

分 类 号：S513.0353[农业科学—作物学]