脑出血模型大鼠PirB和NogoA表达与神经功能缺损  

Expression of PirB and NogoA and neurological deficits in rats with intracranial hemorrhage

作　　者：杨洋 刘梦兰 孟仁亮 陶涛 Yang Yang;Liu Menglan;Meng Renliang;Tao Tao(Department of Neurology,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学附属医院神经内科,四川省泸州市646000

出　　处：《中国组织工程研究》2022年第20期3202-3206,共5页Chinese Journal of Tissue Engineering Research

基　　金：西南医科大学校级基金(2017ZRQN148),项目负责人:杨洋。

摘　　要：背景:NogoA和PirB是髓鞘相关抑制因子及受体,在多种中枢神经系统损伤模型中表达明显增高,且起着抑制轴突再生和阻碍神经功能恢复的作用,脑出血遗留严重持久的神经功能障碍可能与二者有关。目的:通过构建大鼠脑出血模型,探讨PirB和NogoA蛋白表达与神经功能缺损的关系。方法:健康雄性SD大鼠75只,随机分为假手术组(n=25)和脑出血组(n=50),按取材时间点不同分为12 h及1,3,7,14 d组,假手术组大鼠不做任何处理,脑出血组大鼠以自体鼠尾不凝血经改良二次注入法建立脑出血模型。各时间点大鼠进行神经功能缺损评分后深度麻醉断头取脑,采用Western blot检测PirB和NogoA蛋白的定量表达,免疫荧光观察PirB定位表达。结果与结论:①PirB可表达于成年健康大鼠的神经元及星形胶质细胞的胞体和突起;②脑出血组各时间点PirB和NogoA表达较假手术组明显增加,差异有显著性意义(P<0.05),在脑出血后第3天达到高峰,持续至14 d表达仍较高;③脑出血组PirB和NogoA的表达与神经功能评分呈负相关关系;④结果表明,PirB和NogoA均可表达于健康成年大鼠脑组织中,脑出血后PirB和NogoA表达明显上调并抑制轴突再生,从而阻碍神经功能的恢复。BACKGROUND:Neurite outgrowth inhibitor A(NogoA)and paired immunoglobulin-like receptor B(PirB)are myelin-related inhibitors and receptors.They are significantly increased in a variety of central nervous system injury models,and play a role in inhibiting axon regeneration and impeding the recovery of nerve function.Persistent and severe neurological deficits due to intracerebral hemorrhage may be related to both NogoA and PirB.OBJECTIVE:To investigate the protein expression of PirB and NogoA and their important effect on neurological deficits by constructing a rat model of intracranial hemorrhage METHODS:Seventy-five male Sprague-Dawley rats were randomly assigned into a sham surgery group(n=25)and an intracranial hemorrhage group(n=50).According to the time point of sample collection,each group was randomly divided into five subgroups as a 12-hour group,a 1-day group,a 3-day group,a 7-day group,and a 14-day group.Rats in the sham surgery group did not receive any treatment,while a rat intracranial hemorrhage model was established by modified secondary injection with autologous rat tail blood in the intracranial hemorrhage group.After the neurological deficit score was performed at each time point,rats were decapitated under deep anesthesia and brain samples were taken.Western blot was used to detect the quantitative protein expression of PirB and NogoA,and the location expression of PirB was observed by immunofluorescence.RESULTS AND CONCLUSION:PirB could be expressed in neurons and astrocytes of health adult rats.The expression of PirB and NogoA in the intracranial hemorrhage group was significantly higher than that in the sham surgery group at each time point(P<0.05).The expression peaked on the 3^(rd) day after intracranial hemorrhage,and remained relatively high until the 14^(th) day.The expression of PirB and NogoA in the intracranial hemorrhage group was negatively correlated with the neurological deficit scores.These results indicate that PirB and NogoA can be both expressed in the brain tissue of health a

关 键 词：脑出血 PirB NogoA 轴突再生 神经功能缺损 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]