白花蛇舌草和半枝莲乙醇提取物逆转耐阿霉素肝细胞癌的耐药机制研究  

Mechanism of reversal of adriamycin-resistant hepatocellular carcinoma with ethanol extracts of Oldenlandia diffusa roxb and Scutellariae barbatae herba

作　　者：潘云翠 王倩 胡爱艳 徐怡 聂笑宇 张鑫 王守梅[1] 张树辉[1] PAN Yuncui;WANG Qian;HU Aiyan;XU Yi;NIE Xiaoyu;ZHANG Xin;WANG Shoumei;ZHANG Shuhui(Department of Pathology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院病理科,上海200437

出　　处：《上海中医药杂志》2021年第10期52-57,共6页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81172311);上海中医药大学中西医高原学科项目(30304114325)。

摘　　要：目的研究白花蛇舌草(Od)和半枝莲(Sb)乙醇提取物对耐阿霉素肝细胞癌(HepG2/ADM)增殖和凋亡的作用。方法①体外实验:采用CCK-8法检测不同浓度的Od-Sb对HepG2/ADM的影响;克隆形成实验检测Od-Sb对HepG2/ADM克隆形成能力的影响;流式细胞术检测Od-Sb对HepG2/ADM凋亡的影响;Western blot检测Od-Sb对HepG2/ADM中Ki-67、Bcl-2蛋白表达的影响。②体内实验:以裸鼠HepG2/ADM皮下移植瘤为模型,观察Od-Sb对瘤体的作用,并采用免疫组化法检测Ki-67、Bcl-2蛋白的表达。结果①体外实验显示,与对照组比较,Od-Sb可以抑制HepG2/ADM细胞的增殖,抑制HepG2/ADM细胞的克隆形成,促进其凋亡,并下调Ki-67、Bcl-2蛋白的表达(P<0.05)。②体内实验显示,与对照组比较,Od-Sb可以抑制裸鼠HepG2/ADM皮下移植瘤瘤体的生长,下调Ki-67、Bcl-2蛋白的表达(P<0.05,P<0.001)。结论Od-Sb可下调HepG2/ADM中Ki-67、Bcl-2蛋白的表达,抑制HepG2/ADM的生长,并促进其凋亡;Od-Sb通过增殖-凋亡调控机制部分逆转HepG2/ADM的耐药。Objective To study the effects of Oldenlandia diffusa roxb and Scutellariae barbatae herba(Od-Sb)on the proliferation and apoptosis of HepG2/ADM.Methods①In vitro experiment:the effect of different concentrations of Od-Sb on the survival rate of HepG2/ADM was detected by CCK8 assay;the effect of Od-Sb on HepG2/ADM clonal formation ability was detected by cloning formation experiment;the effect of Od-Sb on the apoptosis of HepG2/ADM was detected by flow cytometry;the effect of Od-Sb on the protein expression of Ki-67 and Bcl-2 was detected by Western blot.②In vivo experiment:the subcutaneous transplanted tumor of HepG2/ADM in nude mice was used as a model to observe the inhibitory effect of Od-Sb on the tumor,and the proteins expression of Ki-67 and Bcl-2 was detected by immunohistochemistry.Results①In vitro experiments,it was showed that compared with the control group,Od-Sb could inhibit the proliferation of HepG2/ADM cells,inhibit the formation of HepG2/ADM cells,promote its apoptosis,and down-regulated the expression of Ki-67 and Bcl-2 proteins(P<0.05).②In vivo experiments showed that compared with the control group,Od-Sb inhibited the growth of HepG2/ADM subcutaneous transplanted tumor body,and down-regulated the expression of Ki-67 and Bcl-2 proteins(P<0.05,P<0.001).Conclusion Od-Sb can down-regulate the expression of Ki-67 and Bcl-2 proteins,inhibit the growth of HepG2/ADM and promote its apoptosis.Od-Sb can partially reverse the resistance of HepG2/ADM through the mechanism of proliferation-apoptosis regulation.

关 键 词：白花蛇舌草 半枝莲 肝细胞癌 阿霉素 耐药 模型小鼠 中药研究 

分 类 号：R285.5[医药卫生—中药学]