基于UHPLC-Q Exactive HRMS的轮状病毒肠炎湿热证患儿粪便代谢组学特征研究  

UHPLC-Q Exactive HRMS-based study on fecal metabolomic characteristics of children with rotavirus enteritis of damp-heat syndrome

作　　者：叶青艳[1] 张俞莹 沈健[1] 赵鋆[1] 崔晨[1] 宗南伟 姚婷[1] 陆超元[1] 周君慧[1] 马春艳[1] 周华[3] 杨涛[3] YE Qingyan;ZHANG Yuying;SHEN Jian;ZHAO Jun;CUI Chen;ZONG Nanwei;YAO Ting;LU Chaoyuan;ZHOU Junhui;MA Chunyan;ZHOU Hua;YANG Tao(Department of Pediatrics,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Science and Technology DivisionⅡ,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Institute of Cardiovascular Research,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院儿科,上海201203 [2]上海中医药大学附属曙光医院科技二处,上海201203 [3]上海中医药大学附属曙光医院心血管研究所,上海201203

出　　处：《上海中医药杂志》2021年第11期18-22,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81804141);“十三五”国家科技重大专项(2017ZX10305501-007);上海市中医药领军人才项目(ZY2018-RCPY-1012);上海中医药大学预算内项目(18TS089)。

摘　　要：目的从代谢组学角度探讨轮状病毒肠炎湿热证患儿的粪便代谢组学特征。方法收集20例轮状病毒肠炎湿热证患儿(RV湿热证组)和20例健康儿童(健康组)的粪便样本,采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLC-Q Exactive HRMS)技术采集粪便代谢指纹谱,结合正交偏最小二乘-判别分析(OPLS-DA)的模式识别方法进行数据分析,寻找差异性内源性代谢物。结果OPLS-DA模式识别可有效区分RV湿热证组与健康组,且模型可靠性高。筛选并鉴定出11种差异性代谢物,涉及咖啡因代谢、烟酸盐和烟酰胺代谢、卟啉和叶绿素代谢、不饱和脂肪酸代谢、花生四烯酸代谢、精氨酸和脯氨酸代谢、嘧啶代谢、色氨酸代谢,以及甘氨酸、丝氨酸和苏氨酸代谢等9条代谢通路。结论轮状病毒肠炎湿热证患儿与健康儿童间存在差异性代谢产物,这些代谢化合物的异常表达可能是轮状病毒肠炎湿热证的重要发病机制,也是潜在的干预靶点。Objective To explore the fecal metabolomic characteristics of children with rotavirus enteritis of damp-heat syndrome from the perspective of metabolomics.Methods Fecal samples were collected from 20 children with rotavirus enteritis(RV damp-heat syndrome group)and 20 healthy children,ultra-high performance liquid chromatography coupled with quadrupole-exactive high resolution mass spectrometry(UHPLC-Q Exactive HRMS)was utilized to collect fecal metabolic fingerprints,and orthogonal-partial least squares discriminant analysis(OPLS-DA)pattern recognition approach was introduced for data analysis to identify differential endogenous metabolites.Results OPLS-DA pattern recognition approach can effectively distinguish RV damp-heat syndrome group from healthy group with high reliability.Eleven differential metabolites were screened out and identified,involving 9 metabolic pathways,such as caffeine metabolism,nicotinate and nicotinamide metabolism,porphyrin and chlorophyll metabolism,unsaturated fatty acid metabolism,arachidonic acid metabolism,arginine and proline metabolism,pyrimidine metabolism,tryptophan metabolism,and glycine,serine and threonine metabolism.Conclusion There are differential metabolites in fecal samples between children with rotavirus enteritis of damp-heat syndrome and healthy children.The abnormal expression of these metabolic compounds may be an important pathogenesis of rotavirus enteritis of damp-heat syndrome,and it can also be a potential intervention target.

关 键 词：轮状病毒肠炎 湿热证 代谢组学 超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法 

分 类 号：R725.1[医药卫生—儿科]