机构地区:[1]湖南中医药大学第二附属医院药剂科,湖南长沙410000 [2]湖南中医药大学第二附属医院妇科,湖南长沙410000
出 处:《上海中医药杂志》2021年第11期74-79,共6页Shanghai Journal of Traditional Chinese Medicine
基 金:湖南省中医药科研计划项目(202012)。
摘 要:目的探究消囊促卵丸对多囊卵巢综合征模型大鼠性激素水平的影响及相关作用机制。方法90只6周龄SPF级SD雌性大鼠,随机选取15只作为正常组(灌胃0.9%NaCl溶液)。剩余大鼠建立多囊卵巢综合征模型,建模成功后随机分为模型组(灌胃0.9%NaCl溶液)、阳性药物组(灌胃270 mg/kg二甲双胍)、低剂量组(灌胃3.15 g/kg消囊促卵丸)、中剂量组(灌胃6.30 g/kg消囊促卵丸)、高剂量组(12.60 g/kg消囊促卵丸),每组15只。造模成功后分别给予各组药物干预12 d。观察各组大鼠病理组织学变化,检测空腹胰岛素(FINS)、空腹血糖(FPG)、餐后2 h胰岛素(2 h INS)、餐后2 h血糖(2 h PG)、C-反应蛋白(CRP)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、睾酮(T)、雌二醇(E_(2))、黄体生成素(LH)、促卵泡激素(FSH)水平及卵巢组织中AKT-2、GSK-3β、IRS-1、GLUT-4、PTEN mRNA表达量。分析消囊促卵丸对多囊卵巢综合征大鼠性激素水平的影响机制。结果与模型组比较,高剂量组、中剂量组、低剂量组、阳性药物组腹腔葡萄糖耐量(IPGTT)、炎性因子、T、E_(2)、LH水平,PTEN mRNA表达量降低(P<0.05);与模型组比较,高剂量组、中剂量组、低剂量组、阳性药物组FSH水平,AKT-2、GSK-3β、IRS-1、GLUT-4 mRNA表达量升高(P<0.05);以高剂量组效果最佳。结论消囊促卵丸可能通过作用于PI3K/AKT信号通路,上调AKT-2、GSK-3β、IRS-1、GLUT-4 mRNA表达量,下调PTEN mRNA表达量,促进性功能,减轻炎症反应。Objective To explore the effect of Xiaonang Culuan Wan(XCW)on the levels of sex hormones in rats with polycystic ovary syndrome and its related mechanism of action.Methods Of 90 six-week-old SPF female SD rats,15 were randomly selected as normal group(intragastric administratiion of 0.9%NaCl solution).After successful establishment of polycystic ovary syndrome model,the remaining rats were randomly divided into model group(intragastric administratiion of 0.9%NaCl solution),positive drug group(intragastric administratiion of 270 mg/kg metformin),lowdose group(intragastric administratiion of 3.15 g/kg XCW),medium-dose group(intragastric administratiion of 6.30 g/kg XCW)and high-dose group(intragastric administratiion of 12.60 g/kg XCW),with 15 rats in each group.After successful modeling,each group was given drug intervention for 12 days.The histopathological changes of rats in each group were observed,and the levels of fasting insulin(FINS),fasting plasma glucose(FPG),2-h postprandial insulin(2 h INS),2-h postprandial glucose(2 h PG),C-reactive protein(CRP),interleukin 6(IL-6),tumor necrosis factor-α(TNF-α)、testosterone(T),estradiol(E_(2)),luteinizing hormone(LH),follicle stimulating hormone(FSH)and the expression of AKT-2,GSK-3β、IRS-1,GLUT-4,PTEN mRNA in ovarian tissue were detected,which were used to analyze the mechanism and the effect of XCW on sex hormone levels in rats with polycystic ovary syndrome.Results Compared with the model group,the results of intraperitoneal glucose tolerance test(IPGTT)and levels of inflammatory factors,T,E2,LH and PTEN mRNA expression in the high-dose group,medium-dose group,low-dose group and positive drug group showed lower(P<0.05);Compared with the model group,the level of FSH and levels of AKT-2,GSK-3β,IRS-1 and GLUT-4 mRNA expression in the highdose,medium-dose,low-dose and positive drug groups were higher(P<0.05),and the effect of high-dose group were the best.Conclusion By acting on P13K/AKT signal pathway,XCW can promote sexual function and reduce inflammation mayb
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