Genomic polymorphisms at the crhr2 locus improve feed conversion efficiency through alleviation of hypothalamus-pituitary-interrenal axis activity in gibel carp(Carassius gibelio)  

作　　者：Chuang Shi Qiyong Lou Beide Fu Junyan Jin Jianfei Huang Yao Lu Xia Jin Jiangyan He Gang Zhai Shouqi Xie Jingou Tong Jianfang Gui Zhan Yin 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100864,China

出　　处：《Science China(Life Sciences)》2022年第1期206-214,共9页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China(2018YFD0900404);the Pilot Program A Project from the Chinese Academy of Sciences(XDA24010206);State Key Laboratory of Freshwater Ecology and Biotechnology(2019FBZ05);the National Natural Science Foundation of China(31530077);China Postdoctoral Science Foundation(2020M682527)。

摘　　要：Improvement in fish feed conversion efficiency(FCE)is beneficial for sustaining global food fish supplies.Here,we show that a set of polymorphisms at locus of the corticotropin releasing hormone receptor 2(crhr2),which is involved in hypothalamuspituitary-interrenal(HPI)axis signaling,is associated with improved FCE in farmed allogynogenetic gibel carp strain CAS Ⅲ compared with that in the wild gibel carp strain Dongting(DT).This set of polymorphisms downregulates the expression levels of crhr2 mRNA in the brain and pituitary tissues in gibel carp strain CAS Ⅲ compared with those in strain DT.Furthermore,compromised HPI axis signaling is observed in gibel carp strain CAS Ⅲ,such as decreased α-melanocyte stimulating hormone protein levels,plasma cortisol content,and stress responses.Moreover,enhanced activation of protein kinase B/mammalian target of rapamycin complex 1 signaling observed in the muscle tissue of strain CAS Ⅲ in comparison to that in strain DT indicated elevated anabolic metabolism in strain CAS Ⅲ.Thus,these studies demonstrate that the genetic markers associated with compromised HPI axis signaling,such as crhr2,are potentially useful for genetic selection toward improvement in farmed fish growth and FCE,which would reduce fishmeal consumption and thereby indirectly facilitate sustainable fisheries.

关 键 词：feed conversion efficiency crhr2 stress responses endocrine regulation genetic marker for fish breeding 

分 类 号：S965.117[农业科学—水产养殖]