宫内发育迟缓对猪胎盘细胞增殖、凋亡及抗氧化基因和蛋白表达的影响  

Effects of intrauterine growth retardation on proliferation,apoptosis and antioxidant gene and protein expression of porcine placenta

作　　者：齐丽娜[1] 蒋静乐 陶生祥 张婧菲[1] 张莉莉[1] 王恬[1] QI Lina;JIANG Jingle;TAO Shengxiang;ZHANG Jingfei;ZHANG Lili;WANG Tian(College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,210095,China)

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《南京农业大学学报》2022年第1期141-146,共6页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31772634)。

摘　　要：[目的]本试验旨在探讨宫内发育迟缓(intrauterine growth retardation,IUGR)对猪胎盘细胞增殖、凋亡及抗氧化基因和蛋白表达的影响。[方法]从刚分娩的母猪中选择正常初生重(normal birth weight,NBW)和IUGR仔猪对应的胎盘各5个,分为对照组和宫内发育迟缓组。采用免疫组化法检测胎盘细胞增殖细胞核抗原(PCNA)的表达,采用TUNEL法检测胎盘细胞凋亡,采用荧光定量PCR检测胎盘NRF2、HO-1、NQO1、SOD1、SOD2、GCLC和GCLM基因的表达,用Westen blot(WB)检测胎盘抗氧化蛋白NRF2和HO-1的表达。[结果]对照组可以观察到较强的PCNA染色,宫内发育迟缓组PCNA染色较弱。IUGR组和对照组凋亡细胞指数分别为19.63%与12.56%,IUGR组细胞凋亡率明显高于对照组(P<0.05)。RT-qPCR结果表明,与对照组相比,宫内发育迟缓猪胎盘NRF2、NQO1、SOD1、SOD2、GCLC、GCLM mRNA的表达量显著降低(P<0.05),HO-1 mRNA的表达在正常猪胎盘和宫内发育迟缓猪胎盘组间差异不显著(P>0.05)。WB结果显示,对照组与宫内发育迟缓组NRF2和HO-1蛋白表达量差异不显著(P>0.05)。[结论]IUGR会降低猪胎盘细胞增殖,增加猪胎盘细胞凋亡且降低猪胎盘抗氧化基因的表达。[Objectives]This experiment was conducted to study the effects of intrauterine growth retardation(IUGR)on proliferation,apoptosis and antioxidant genes and protein expression of porcine placenta.[Methods]In this experiment,the placentas of normal birth weight and IUGR piglets were selected from newly delivered sows,and they were divided into the control group and IUGR group.Each group contained five replicates.Immunohistochemistry(IHC)was used to detect the PCNA expression in the placental cells.The apoptosis of placental cells was detected by TUNEL.RT-qPCR was applied to detect the mRNA expression of NRF2,HO-1,NQO1,SOD1,SOD2,GCLC and GCLM.Western blot(WB)was applied to determine the protein expression of NRF2 and HO-1.[Results]The results showed as follows:Stronger PCNA immunostaining was observed in the control group,while weaker PCNA staining was observed in the IUGR group.The percentage of apoptotic cells was 12.56%in the control group,whereas that of the IUGR group was 19.63%,the apoptosis rate of the IUGR group was significantly higher than that of the control group(P<0.05).The results of RT-PCR showed that compared with the control group,the mRNA expression of NRF2,NQO1,SOD1,SOD2,GCLC and GCLM in placenta of the IUGR group was significantly decreased(P<0.05).While the expression of HO-1 mRNA showed no significant difference between the control group and the IUGR group(P>0.05).The results of WB showed that the protein expression levels of NRF2 and HO-1 in the control group and the IUGR group were not significantly different(P>0.05).[Conclusions]Intrauterine growth retardation could inhibit the proliferation of porcine placental cells,increase apoptosis and decrease the expression of antioxidant genes and proteins in porcine placenta.

关 键 词：宫内发育迟缓 胎盘 增殖 凋亡 抗氧化 

分 类 号：S828[农业科学—畜牧学]