潜阳育阴颗粒对自发性高血压大鼠肾脏足细胞损伤的影响及其机制研究  

Mechanism of Qianyang Yuyin Granule on Renal Podocytes Injury in Spontaneously Hypertensive Rats

作　　者：李婕 马思齐 许骏尧 郑亚威 王艺璇 李海涛[2] LI Jie;MA Si-qi;XU Jun-yao;ZHENG Ya-wei;WANG Yi-xuan;LI Hai-tao(The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China;School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;The First School of Clinical Medine, Nanjing University of Chinese Medicine, Nanjing 210023, China)

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]南京中医药大学药学院,江苏南京210023 [3]南京中医药大学第一临床医学院,江苏南京210023

出　　处：《南京中医药大学学报》2022年第1期69-76,共8页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(81904113);江苏省自然科学基金青年基金项目(BK20181095)。

摘　　要：目的探讨潜阳育阴颗粒(QYYY)对高血压肾损害的治疗作用及其作用机制。方法以自发性高血压大鼠(SHR)为动物模型,潜阳育阴颗粒(低、中、高剂量)为干预,HE染色及Masson染色观察各组大鼠肾脏病理情况,Western Blot检测TRPC6及足细胞损伤相关蛋白Desmin、Nephrin和Podocin的表达。细胞实验用潜阳育阴含药血清低、中、高剂量(QYYYL、QYYYM、QYYYH)干预血管紧张素Ⅱ(AngⅡ)诱导的大鼠足细胞损伤模型,Western Blot检测TRPC6及足细胞损伤相关蛋白的表达。免疫荧光观察TRPC6、Desmin的表达。结果HE染色及Masson染色观察到潜阳育阴颗粒能明显改善SHR的肾损害,与模型组相比,QYYY能明显降低TRPC6、Desmin,升高Nephrin、Podocin表达水平及改善AngⅡ所诱导的足细胞损伤(P<0.05,P<0.01)。结论QYYY可以改善高血压肾损害,其机制可能与TRPC6及足细胞损伤相关蛋白的表达有关。OBJECTIVE To investigate the therapeutic effect of Qianyang Yuyin Granule(QYYY)on hypertensive kidney injury.METHODS spontaneously hypertensive rats(SHR)were chosen as the animal model to evaluate the QYYY therapeutic effect.HE and Masson staining were used to observe the kidney pathology in each treatment group.The expression of TRPC6 and podocyte damage-related proteins Desmin,Nephrin and Podocin was determined by Western blot assay.In addition,low,medium and high doses(QYYYL,QYYYM,QYYYH)of Qianyang Yuyin drug-containing serum were used to treat an in vitro rat podocyte injury model induced by angiotensinⅡ(AngⅡ).Similarly,Western blot and immunofluorescence assays were used to determine the expression of TRPC6 and podocyte damage-related proteins.RESULTS HE and Masson staining indicated that QYYY treatment markedly alleviated the kidney damage in SHR,which correlates with significant reduction of TRPC6 and Desmin,and induction of Nephrin and Podocin,as well as the improvement of AngⅡ-induced podocyte injury.CONCLUSION QYYY can ameliorate kidney and podocyte damage,and reduce hypertensive kidney damage.

关 键 词：潜阳育阴颗粒 高血压肾损害 足细胞损伤 

分 类 号：R285.5[医药卫生—中药学]