透明草金鱼的皮肤转录组分析  

Transcriptome Analysis of Transparent Skin in Grass Goldfish Carassius auratus

作　　者：刘肖莲[1] 李春艳[1] 白晓慧[1] 郝爽[1] 刘国山[1] 刘克明[1] 马林[1] 姜巨峰[1] LIU Xiaolian;LI Chunyan;BAI Xiaohui;HAO Shuang;LIU Guoshan;LIU Keming;MA Lin;JIANG Jufeng(Tianjin Ornamental Fish Technology and Engineering Center, Tianjin Fisheries Research Institute, Tianjin 300221, China)

机构地区：[1]天津市水产研究所,天津市观赏鱼技术工程中心,天津300221

出　　处：《水产科学》2022年第1期110-115,共6页Fisheries Science

基　　金：天津市科技计划项目(18ZXBFNC00160);天津市水产产业技术体系观赏鱼设施养殖项目(ITTFRS2017016).

摘　　要：采用Illumina HiSeq技术对体长(45.00±5.42)mm的透明草金鱼和不透明草金鱼皮肤组织透明性状相关基因进行转录组测序。6个组织共产生309364078条过滤序列,组装后获得72083个unigene,平均长度为1321 bp。DESeq分析共筛选出181个差异表达基因(DEGs),其中上调基因66个、下调基因115个。GO功能注释发现,差异表达基因主要与微管、肌球蛋白复合物、蛋白质绑定、肌动蛋白结合、鸟嘌呤核苷酸结合、色素沉着、色素颗粒运输、中间纤维等相关。KEGG通路富集分析显示,差异基因富集在MAPK信号通路、肌动蛋白细胞骨架调节通路、嘌呤代谢通路、内质网的蛋白质加工通路等。此外,从72083个unigene中查找出38775个微卫星位点,出现频率最高的为单碱基重复(53.95%)和二碱基重复(24.45%)。本试验结果将为草金鱼透明性状相关基因的挖掘与功能研究提供基础信息。Two libraries of transparent group and normal group were constructed and sequenced in grass goldfish Carassius auratus with body length of(45.00±5.42)mm by Illumina high-throughput sequencing technology to explore the functional genes related to transparent trait of grass goldfish.A total of 309364078 clean reads were generated from six skin tissues,and assembled into 72083 unigenes with an average length of 1321 bp.DESeq analysis identified 181 differentially expressed genes(DEGs)between the two groups,among which 66 genes were up-regulated and 115 genes were down-regulated.Gene ontology functional enrichment analysis showed that DEGs were mostly involved in microtubule,microtubule cytoskeleton organization,intermediate filament,myosin complex,protein binding,guanyl ribonucleotide binding,pigmentation,and pigment granule transport.KEGG pathway analysis revealed that DEGs were mainly distributed in MAPK signaling pathway,regulation of actin cytoskeleton,purine metabolism,and protein processing in endoplasmic reticulum.In addition,38775 microsatellite loci were screened out from 72083 unigenes with the highest frequency of mono-nucleotide repeats(53.95%)and followed by di-nucleotide repeats(24.45%).The finding provides basic information for exploring new genes related to transparent trait and their functional analysis of grass goldfish C.auratus.

关 键 词：草金鱼 透明性状 转录组测序 差异表达基因 

分 类 号：Q959.4[生物学—动物学]