一株氨化芽孢细菌的分离鉴定及氨化培养基优化  

Isolation and Identification of an Ammonifying Spore Bacterium and Optimization of Ammonifying Medium

作　　者：朱欣乐 陈思宇 杨正 刘晶[1] 黄元昊 彭英杰 兰时乐[1] ZHU Xinle;CHEN Siyu;YANG Zheng;LIU Jing;HUANG Yuanhao;PENG Yingjie;LAN Shile(College of Bioscience and Biotechnology,Hunan Agricultural Univeisity,Changsha 410128, China)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《湖南生态科学学报》2022年第1期34-43,共10页Journal of Hunan Ecological Science

基　　金：湖南省自科基金省市联合基金(2020JJ6058)。

摘　　要：【目的】降低养殖废水中有机氮含量。【方法】从精养池塘底泥中分离得到一株具有高效氨化能力的芽孢杆菌,命名为ZXL-7。通过形态学观察、生理生化实验和16S rDNA序列分析进行菌株鉴定;利用单因素试验研究培养基组成对菌株ZXL-7氨化效果的影响。根据单因素试验结果,采用响应面法对影响氨化效果较大的因素进行了优化。【结果】结果表明,高效氨化能力菌株ZXL-7为巨大芽孢杆菌(Bacillus megaterium)。优化的氨化培养基组成为:丁二酸钠28.04 g/L,酵母提取物8.73 g/L,KH_(2)PO_(4)2.05 g/L,MgSO_(4)·7H_(2)O 1.58 g/L,MnSO_(4)·H_(2)O 0.61 g/L,NaCl 3.00 g/L。【结论】菌株ZXL-7对降低养殖废水中的有机氮含量具有一定的应用价值。【Objective】It is to reduce the content of organic nitrogen in aquaculture wastewater.【Method】A Bacillus with high ammoniation ability which was named ZXL-7 was isolated from the sediment of intensive culture pond.Phyziological biochemical experiments.The effect of medium composition on ammoniation of strain ZXL-7 was studied by single-factor test.Based on the single-factor test results,the factors affecting the ammoniation were optimized by response surface method.The results showed that strainZXL-7 was identified as Bacillus megaterium.【Result】The optimized ammoniation medium were:Sodium succinate 28.04 g/L,Yeast extract 8.73 g/L,KH_(2)PO_(4)2.05 g/L,MgSO_(4)·7H_(2)O 1.58 g/L,MnSO_(4)·H_(2)O 0.61 g/L and NaCl 3 g/L.【Conclusion】Strain ZXL-7 has certain application value in reducing the content of organic nitrogen in aquaculture wastewater.

关 键 词：氨化细菌 养殖废水 BOX-BEHNKEN设计 培养基优化 

分 类 号：Q939.9[生物学—微生物学]